Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4A60_RS13665 Genome accession   NZ_CP015222
Coordinates   2553702..2554319 (-) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain HRBS-10TDI13     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2548702..2559319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4A60_RS13640 (A4A60_13650) rpsT 2549004..2549270 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  A4A60_RS13645 (A4A60_13655) holA 2549285..2550328 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  A4A60_RS23040 - 2550368..2550517 (-) 150 WP_003229985.1 hypothetical protein -
  A4A60_RS13650 (A4A60_13660) yqzM 2550558..2550692 (+) 135 WP_003229983.1 YqzM family protein -
  A4A60_RS13655 (A4A60_13665) comEC 2550732..2553062 (-) 2331 WP_069837640.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A4A60_RS13660 (A4A60_13670) comEB 2553066..2553635 (-) 570 WP_003229978.1 ComE operon protein 2 -
  A4A60_RS13665 (A4A60_13675) comEA 2553702..2554319 (-) 618 WP_046160608.1 competence protein ComEA Machinery gene
  A4A60_RS13670 (A4A60_13680) comER 2554403..2555224 (+) 822 WP_032726195.1 late competence protein ComER -
  A4A60_RS13675 (A4A60_13685) yqeM 2555290..2556033 (-) 744 WP_069837641.1 class I SAM-dependent methyltransferase -
  A4A60_RS13680 (A4A60_13690) rsfS 2556030..2556386 (-) 357 WP_014480315.1 ribosome silencing factor -
  A4A60_RS13685 (A4A60_13695) yqeK 2556404..2556964 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  A4A60_RS13690 (A4A60_13700) nadD 2556954..2557523 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  A4A60_RS13695 (A4A60_13705) yhbY 2557535..2557825 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  A4A60_RS13700 (A4A60_13710) aroE 2557819..2558661 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=177667 A4A60_RS13665 WP_046160608.1 2553702..2554319(-) (comEA) [Bacillus subtilis strain HRBS-10TDI13]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=177667 A4A60_RS13665 WP_046160608.1 2553702..2554319(-) (comEA) [Bacillus subtilis strain HRBS-10TDI13]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment