Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   A4H00_RS05770 Genome accession   NZ_CP015196
Coordinates   1120019..1121338 (+) Length   439 a.a.
NCBI ID   WP_067088101.1    Uniprot ID   -
Organism   Streptococcus marmotae strain HTS5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1105953..1128239 1120019..1121338 within 0


Gene organization within MGE regions


Location: 1105953..1128239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4H00_RS05715 - 1105953..1110347 (+) 4395 WP_067088085.1 PolC-type DNA polymerase III -
  A4H00_RS05720 - 1110450..1111964 (-) 1515 WP_067088087.1 ABC transporter permease/substrate-binding protein -
  A4H00_RS05725 - 1111974..1112690 (-) 717 WP_067088090.1 ABC transporter ATP-binding protein -
  A4H00_RS05730 - 1112950..1114191 (+) 1242 WP_067088091.1 aminopeptidase -
  A4H00_RS05735 - 1114220..1114450 (+) 231 WP_067088093.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  A4H00_RS05740 - 1114579..1116131 (+) 1553 Protein_1093 IS1182 family transposase -
  A4H00_RS11475 - 1116172..1117219 (+) 1048 WP_099092189.1 IS630 family transposase -
  A4H00_RS05755 - 1117384..1118640 (+) 1257 WP_067085967.1 ISL3 family transposase -
  A4H00_RS05760 - 1118780..1119235 (-) 456 WP_067088097.1 SP_0198 family lipoprotein -
  A4H00_RS05765 - 1119302..1119952 (-) 651 WP_067088099.1 YigZ family protein -
  A4H00_RS05770 comFA/cflA 1120019..1121338 (+) 1320 WP_067088101.1 DEAD/DEAH box helicase Machinery gene
  A4H00_RS05775 comFC/cflB 1121304..1121969 (+) 666 WP_067088103.1 ComF family protein Machinery gene
  A4H00_RS05780 hpf 1122046..1122585 (+) 540 WP_067088106.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A4H00_RS05785 pbp3 1122633..1123865 (-) 1233 WP_067091512.1 D-alanyl-D-alanine carboxypeptidase PBP3 -
  A4H00_RS05790 - 1123967..1124167 (-) 201 WP_067088107.1 hypothetical protein -
  A4H00_RS05795 oppA 1124253..1126037 (+) 1785 WP_067088109.1 oligopeptide ABC transporter substrate-binding protein Regulator
  A4H00_RS05800 - 1126294..1127295 (+) 1002 WP_067088111.1 ABC transporter ATP-binding protein -
  A4H00_RS05805 - 1127295..1128239 (+) 945 WP_067088113.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50076.69 Da        Isoelectric Point: 8.2223

>NTDB_id=177441 A4H00_RS05770 WP_067088101.1 1120019..1121338(+) (comFA/cflA) [Streptococcus marmotae strain HTS5]
MTDMTNYYGRLFTHHQLTETERLQAKTIPSQTPHYTCFRCATLFEEKHCLPNGALYCRECILLGRVRTDEDLYYFPQQPF
PSQRSLKWEGELTTWQQEISDGLRQNFKERKATLVHAVTGAGKTEMIYQLVADVIDGGGAVCLASPRIDVCIELYKRLQK
DFACPISLLHGTSEPYFRTPLVIATTHQLLKFYRAFDLILIDEVDAFPYVDNPMLYRAVENAVKADGVIMFLTATSTDEL
EKKVQSGTLHRLRLPRRFHGNPLVVPQKTWLSQFEAQLKKKKITPKLLSKIAEQRKTGYPLLIFLPEIRMGQSFAQLLEK
DFQDQGIGFVSSQTDNRLEIVEQFRRREIGILVTTTILERGVTFPCVDVFVVQANHALYTSSSLIQIAGRVGRSMERPTG
DLLFFHDGSNRAIEKAIREIRLMNKEAGYDSVPTVPASS

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=177441 A4H00_RS05770 WP_067088101.1 1120019..1121338(+) (comFA/cflA) [Streptococcus marmotae strain HTS5]
ATGACAGATATGACAAATTACTATGGGAGATTGTTTACCCACCATCAATTGACGGAAACAGAGCGACTGCAAGCTAAAAC
GATACCAAGCCAAACACCGCATTATACGTGTTTTCGCTGTGCAACTCTATTTGAAGAAAAGCACTGCTTGCCAAATGGAG
CTCTGTACTGTCGAGAATGTATACTATTGGGACGTGTACGGACAGATGAAGATTTGTACTATTTTCCTCAGCAACCATTT
CCTTCTCAACGCTCTCTCAAGTGGGAAGGTGAATTAACCACTTGGCAGCAAGAAATTTCAGATGGACTACGACAGAATTT
CAAAGAAAGAAAAGCTACTTTAGTACATGCAGTAACAGGAGCTGGAAAGACAGAGATGATTTACCAGCTGGTTGCTGATG
TGATTGATGGAGGTGGCGCAGTTTGCTTGGCCAGTCCGCGGATTGACGTTTGTATCGAATTGTATAAGAGGTTGCAGAAA
GATTTTGCCTGTCCAATTAGCCTCTTACATGGGACCTCGGAGCCATATTTTCGGACACCTTTAGTTATTGCGACCACACA
TCAATTGCTCAAATTTTATCGAGCTTTTGATTTGATTTTGATTGATGAAGTGGATGCTTTTCCCTATGTCGACAATCCTA
TGCTGTATCGTGCAGTAGAAAATGCCGTTAAAGCAGATGGAGTAATCATGTTTTTGACGGCAACTTCGACAGATGAATTA
GAGAAAAAAGTTCAATCAGGAACCCTTCATCGTCTGCGTTTACCACGGCGGTTTCATGGTAACCCACTAGTTGTTCCACA
AAAGACGTGGCTGTCACAGTTTGAAGCACAGCTAAAAAAGAAAAAGATAACTCCAAAATTACTTAGTAAGATAGCAGAGC
AACGAAAAACAGGATACCCACTGCTGATTTTTTTGCCTGAGATTCGTATGGGACAATCTTTTGCCCAGTTGCTGGAAAAG
GATTTCCAAGATCAAGGGATTGGCTTTGTGTCCAGTCAGACAGATAATCGATTGGAAATCGTTGAGCAATTTCGACGCCG
GGAAATCGGGATTCTTGTCACAACAACCATCTTAGAACGAGGAGTGACCTTTCCTTGTGTAGATGTGTTTGTTGTACAAG
CTAACCACGCTCTCTACACCTCTTCTTCGCTCATTCAGATTGCTGGAAGGGTAGGGCGCAGTATGGAGAGACCAACAGGA
GATTTGCTCTTTTTTCACGATGGAAGCAACCGTGCGATTGAAAAAGCTATTCGGGAAATTCGACTAATGAACAAGGAGGC
GGGATATGACAGTGTGCCTACTGTGCCAGCAAGCTCTTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-166)

Helicase conserved C-terminal domain (Helicase_C)

(287-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

65.493

97.039

0.636
  comFA/cflA Streptococcus pneumoniae Rx1

65.258

97.039

0.633
  comFA/cflA Streptococcus pneumoniae D39

65.258

97.039

0.633
  comFA/cflA Streptococcus pneumoniae R6

65.258

97.039

0.633
  comFA/cflA Streptococcus pneumoniae TIGR4

65.258

97.039

0.633
  comFA/cflA Streptococcus mitis SK321

64.789

97.039

0.629
  comFA Lactococcus lactis subsp. cremoris KW2

52.03

89.749

0.467
  comFA Latilactobacillus sakei subsp. sakei 23K

39.394

97.722

0.385

Multiple sequence alignment