Detailed information NTG0177210

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: BE959_RS12085
• Genome accession: NZ_CP018965
• Coordinates: 2133235..2133894 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain Ecol_517
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 2128235..2138894

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE959_RS12055 (BE959_12350)	ygiN	2129136..2129450 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
BE959_RS12060 (BE959_12355)	mdaB	2129481..2130062 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BE959_RS26755 (BE959_12360)	-	2130312..2130791 (+)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
BE959_RS26760 (BE959_12365)	-	2130794..2131504 (+)	711	WP_000834030.1	hypothetical protein	-
BE959_RS12075 (BE959_12370)	ygiZ	2131511..2131843 (+)	333	WP_000914690.1	DUF2645 family protein	-
BE959_RS12080 (BE959_12375)	qseC	2131889..2133238 (-)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
BE959_RS12085 (BE959_12380)	ciaR	2133235..2133894 (-)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
BE959_RS12090 (BE959_12385)	ygiW	2134046..2134438 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BE959_RS12095 (BE959_12390)	ygiV	2134491..2134973 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
BE959_RS12100 (BE959_12395)	mqsR	2135178..2135474 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
BE959_RS12105 (BE959_12400)	mqsA	2135476..2135871 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
BE959_RS12110 (BE959_12405)	ygiS	2136004..2137611 (+)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=177210 BE959_RS12085 WP_001221502.1 2133235..2133894(-) (ciaR) [Escherichia coli strain Ecol_517]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=177210 BE959_RS12085 WP_001221502.1 2133235..2133894(-) (ciaR) [Escherichia coli strain Ecol_517]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365