Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   BUN12_RS04375 Genome accession   NZ_CP018902
Coordinates   785610..787658 (+) Length   682 a.a.
NCBI ID   WP_013352228.1    Uniprot ID   A0A9P1JH06
Organism   Bacillus amyloliquefaciens strain HK1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 780610..792658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUN12_RS04345 spoVM 781254..781334 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  BUN12_RS04350 (BUN12_0849) rpmB 781411..781599 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  BUN12_RS04355 (BUN12_0850) - 781859..782221 (+) 363 WP_013352224.1 Asp23/Gls24 family envelope stress response protein -
  BUN12_RS04360 (BUN12_0851) - 782237..783904 (+) 1668 WP_013352225.1 DAK2 domain-containing protein -
  BUN12_RS04365 (BUN12_0852) sdaAB 784047..784709 (+) 663 WP_013352226.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  BUN12_RS04370 (BUN12_0853) sdaAA 784730..785632 (+) 903 WP_013352227.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  BUN12_RS04375 (BUN12_0854) recG 785610..787658 (+) 2049 WP_013352228.1 ATP-dependent DNA helicase RecG Machinery gene
  BUN12_RS04380 (BUN12_0855) fapR 787765..788331 (+) 567 WP_013352229.1 transcription factor FapR -
  BUN12_RS04385 (BUN12_0856) plsX 788345..789343 (+) 999 WP_013352230.1 phosphate acyltransferase PlsX -
  BUN12_RS04390 (BUN12_0857) fabD 789362..790315 (+) 954 WP_013352231.1 ACP S-malonyltransferase -
  BUN12_RS04395 (BUN12_0858) fabG 790308..791048 (+) 741 WP_013352232.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  BUN12_RS04400 (BUN12_0859) acpP 791133..791366 (+) 234 WP_003154310.1 acyl carrier protein -
  BUN12_RS04405 (BUN12_0860) rncS 791505..792254 (+) 750 WP_013352233.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 77907.35 Da        Isoelectric Point: 6.9229

>NTDB_id=176649 BUN12_RS04375 WP_013352228.1 785610..787658(+) (recG) [Bacillus amyloliquefaciens strain HK1]
MKHHQQTSIAEIKGIGPETEKTLQELGIYDISDLLNYFPYRYDDYELRDLEEVKHEERVTVEGKVHSEPSLTYYGKKRNR
LTFRVLVGNYLITAVCFNRPYLKKKLTLGSVVTISGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
KEALKHHLDNIADPLPEKLRISYKLPDYKQALQTMHQPETRESLQQARRRFVYEEFLLFQLKMQAFRKAEREQSKGISHV
FPAEKLAAFTASLPFSLTNAQMRVLREITADLTSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFANEDVNIALLTSSVKGKRRRELLERLALGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVDQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKQIETYWVKHDMLERILAFIEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSEVYRGKWNVGLMHGKLHSDEKDQVMREFSANQCQVLVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGDHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVSSEAFWKDDEYRMLRAQLLSSGVLEGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=176649 BUN12_RS04375 WP_013352228.1 785610..787658(+) (recG) [Bacillus amyloliquefaciens strain HK1]
GTGAAACACCATCAGCAAACTAGTATAGCGGAGATTAAGGGCATTGGGCCGGAAACAGAAAAAACATTGCAAGAATTGGG
TATCTATGACATTTCTGATCTTCTGAATTATTTCCCTTACCGTTATGATGACTATGAGCTGAGGGATTTGGAAGAAGTTA
AACATGAAGAAAGAGTTACAGTAGAAGGGAAGGTTCATTCAGAACCTTCTCTTACCTATTACGGCAAAAAACGAAACAGG
CTGACATTCAGGGTGCTTGTCGGCAATTATTTAATTACCGCGGTCTGCTTTAACCGTCCGTACTTAAAAAAGAAACTGAC
GCTCGGTTCTGTCGTCACGATATCCGGGAAATGGGATAAACACAGGCAGACCATTTCTGTGCAGGAATTAAAAAACGGGC
CTCATCAAGAAGATAAAAGCATTGAGCCTGTTTATTCCGTTAAAGAAAACGTCACCGTTAAAATGATGAGGCGGTTTATT
AAGGAAGCTTTGAAGCACCATTTGGACAATATTGCCGATCCGCTTCCTGAAAAATTGAGAATCAGTTACAAGCTGCCTGA
TTACAAACAAGCCCTGCAGACGATGCACCAGCCGGAAACGAGGGAATCGTTACAGCAGGCAAGACGCAGGTTTGTTTACG
AAGAATTCTTATTATTTCAGCTGAAAATGCAGGCGTTCCGTAAAGCGGAAAGAGAACAGTCAAAAGGCATCAGCCATGTG
TTTCCGGCTGAAAAGCTCGCCGCTTTTACAGCCAGCCTTCCGTTTTCGCTCACGAACGCACAGATGCGCGTGCTCCGTGA
AATTACCGCTGATTTGACATCTCCTTACCGTATGAACCGTCTGCTGCAAGGCGATGTCGGGTCAGGGAAAACGGCCGTCG
CCGCCATCGCTTTGTACGCTGCGATCCTGTCGGGGTATCAGGGGGCTTTAATGGTGCCGACTGAAATTCTGGCCGAACAG
CATGCCGACTCTCTCGTATCGTTATTTGCAAATGAAGATGTGAACATCGCGCTTTTGACGAGTTCTGTTAAAGGAAAGCG
GCGCAGAGAGCTTTTGGAGCGGCTTGCTCTCGGAGAAATTGATATTTTAGTAGGGACCCATGCTTTAATCCAGGATGAAG
TGGAATTTAAAGCGCTGAGCCTTGTCATTACGGACGAGCAGCATCGGTTCGGGGTCGACCAGCGCAAAAAACTCAGGAAT
AAAGGCCAGGACCCGGATGTGCTGTTTATGACAGCCACCCCGATACCGAGAACATTAGCCATTACCGTCTTCGGAGAAAT
GGATGTTTCCGTTATAGATGAAATGCCCGCGGGACGAAAACAAATCGAAACGTATTGGGTGAAACACGACATGCTGGAGC
GGATTTTGGCTTTTATTGAAAAAGAGCTGAAGCAGGGAAGGCAGGCCTATATTATCTGCCCGCTCATTGAAGAGTCGGAT
AAGCTGGATGTTCAGAATGCGATTGACGTATATAACATGCTGTCTGAGGTTTACCGCGGCAAATGGAATGTCGGTCTGAT
GCACGGGAAGCTCCATTCTGATGAAAAAGATCAGGTGATGAGAGAATTCAGCGCCAATCAATGTCAGGTTCTTGTGTCAA
CAACTGTCGTAGAAGTCGGGGTGAACGTGCCGAATGCGACGATTATGGTCATCTATGACGCCGACCGTTTCGGGCTGTCT
CAGCTCCATCAGCTCCGCGGCCGGGTCGGACGGGGAGACCATCAGTCTTTCTGCATCTTGATGGCCGATCCGAAATCCGA
AACCGGGAAAGAACGGATGAGAATCATGTCGGAGACAAACGACGGCTTTGAACTGTCTGAAAAGGACCTTGAGCTGCGCG
GTCCCGGCGATTTCTTCGGGAAAAAACAGAGCGGTATGCCCGAGTTCAAGGTGGCGGATATGGTTCATGACTACAGAGCT
TTGGAAACGGCCCGCCAGGATGCGGCCAATCTGGTGTCATCAGAGGCTTTTTGGAAAGATGACGAGTACCGCATGCTCCG
CGCTCAACTGCTCTCAAGCGGTGTCTTAGAAGGGGAGAAATTAAGCTGA

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(11-164)

DEAD/DEAH box helicase (DEAD)

(277-415)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1JH06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

90.029

100

0.9
  recG/mmsA Streptococcus pneumoniae R6

49.776

98.387

0.49
  recG/mmsA Streptococcus pneumoniae R36A

49.776

98.387

0.49
  recG Neisseria meningitidis strain C311

38.897

95.748

0.372

Multiple sequence alignment