Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   BUN12_RS04325 Genome accession   NZ_CP018902
Coordinates   776952..778895 (+) Length   647 a.a.
NCBI ID   WP_013352220.1    Uniprot ID   A0A9P1NHU6
Organism   Bacillus amyloliquefaciens strain HK1     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 771952..783895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUN12_RS04300 (BUN12_0840) def 772326..772808 (+) 483 WP_013352215.1 peptide deformylase -
  BUN12_RS04305 (BUN12_0841) fmt 772813..773766 (+) 954 WP_013352216.1 methionyl-tRNA formyltransferase -
  BUN12_RS04310 (BUN12_0842) rsmB 773753..775096 (+) 1344 WP_013352217.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  BUN12_RS04315 (BUN12_0843) rlmN 775100..776191 (+) 1092 WP_013352218.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BUN12_RS04320 (BUN12_0844) - 776197..776958 (+) 762 WP_013352219.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  BUN12_RS04325 (BUN12_0845) stkP 776952..778895 (+) 1944 WP_013352220.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  BUN12_RS04330 (BUN12_0846) rsgA 778910..779800 (+) 891 WP_013352221.1 ribosome small subunit-dependent GTPase A -
  BUN12_RS04335 (BUN12_0847) rpe 779802..780455 (+) 654 WP_013352222.1 ribulose-phosphate 3-epimerase -
  BUN12_RS04340 (BUN12_0848) - 780523..781167 (+) 645 WP_013352223.1 thiamine diphosphokinase -
  BUN12_RS04345 spoVM 781254..781334 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  BUN12_RS04350 (BUN12_0849) rpmB 781411..781599 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  BUN12_RS04355 (BUN12_0850) - 781859..782221 (+) 363 WP_013352224.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71540.31 Da        Isoelectric Point: 5.1147

>NTDB_id=176648 BUN12_RS04325 WP_013352220.1 776952..778895(+) (stkP) [Bacillus amyloliquefaciens strain HK1]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYITAHGPLHPKEALSVMKQIVSAIAHAHHNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKMPFDGESAVSIALKHLQAETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAIAALQAETPAETDDKEQPSA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKEVSVPDVRGMKYEKAEALLEKNGLQADPDMTDIEDEKIEEGLMV
KTDPKAGSAVKEGSSVKLYKSIGKPKTQLIDVKGRPIDDAKKTLKDKGFKHVNVKEENDDSAAGTVIDQNPSAGTDMVVS
DDKVSLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKEAHSDDVPEGQVMKQEPAAGTAVKPGSDVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=176648 BUN12_RS04325 WP_013352220.1 776952..778895(+) (stkP) [Bacillus amyloliquefaciens strain HK1]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCGAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTTGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGACATTTATTATATTGTCATGGAATATGTGGAAGGCATGACGTTAAAGGAATACATAACCGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCTGTTATGAAGCAGATCGTTTCAGCCATCGCCCATGCCCATCATAACCATATCGTGCACCGGG
ATATCAAACCGCACAATATTTTGATAGATCATTTAGGCCATATCAAGGTGACGGATTTCGGCATCGCCACAGCCCTCACT
TCGACGACAATTACACATACGAATTCTGTGCTCGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCGGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGCAAAATGCCTTTTGACGGAGAAT
CAGCCGTCAGCATTGCGTTAAAGCATCTGCAAGCAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTGGAAAACATTATTTTAAAGGCAACCGCCAAAGATCCGTTTCACCGCTATGAAAGCGCAGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTCTCCGTGCAGGATGATGAGGAAATGACGAAAGCGATTC
CCGTTATTACTGACGGTGCGATAGCCGCGCTTCAGGCGGAAACGCCGGCAGAGACGGATGATAAAGAACAGCCGTCCGCC
AAAAAGAAGAAACGGAAATGGCCGTGGGTTTTGCTGGCTGTCTGCTTTATTTTCATAATGGCGGCTGTTCTTGCAGTCAC
CGTGTTCCCGTCTTTGTTTATGCCCAAAGAGGTAAGCGTCCCCGATGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGCCTTCAAGCGGATCCTGATATGACGGACATTGAAGATGAAAAAATCGAAGAGGGATTAATGGTC
AAAACTGATCCGAAAGCGGGTTCTGCCGTTAAAGAAGGCTCTTCTGTAAAGCTGTACAAAAGCATCGGGAAACCGAAAAC
CCAGCTGATTGACGTGAAAGGCCGGCCGATCGATGATGCGAAAAAAACGCTTAAGGATAAAGGCTTCAAACATGTCAATG
TAAAAGAAGAGAACGATGACAGTGCGGCTGGCACCGTAATTGATCAGAACCCATCAGCCGGAACCGACATGGTCGTAAGC
GATGATAAAGTCAGCTTAACAGTCAGCCTCGGACCGGCGGACGTTACGCTGAGAGACTTAAAAACATACAGTAAAGAAGC
GGCTTCCGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAGGAAGCTCACTCAGATGACGTGCCTGAGGGACAGG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTGATGTTGAAGTAACGTTTTCGCTCGGGCCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATCCCCTACGAACCCGAACATGAAGGGGACGAGCTCGAAGTGCA
AATCGCCATCGATGATAAAGACCACAGCATCTCTGATACGTATGACTCGTTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1NHU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366