Detailed information NTG0176064

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: A3772_RS13815
• Genome accession: NZ_CP015004
• Coordinates: 2620118..2620735 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SZMC 6179J isolate B23
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2615118..2625735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A3772_RS13790 (A3772_13790)	rpsT	2615420..2615686 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
A3772_RS13795 (A3772_13795)	holA	2615701..2616744 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
A3772_RS22745	-	2616784..2616933 (-)	150	WP_003229985.1	hypothetical protein	-
A3772_RS13800 (A3772_13800)	yqzM	2616974..2617108 (+)	135	WP_003229983.1	YqzM family protein	-
A3772_RS13805 (A3772_13805)	comEC	2617148..2619478 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A3772_RS13810 (A3772_13810)	comEB	2619482..2620051 (-)	570	WP_003229978.1	ComE operon protein 2	-
A3772_RS13815 (A3772_13815)	comEA	2620118..2620735 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
A3772_RS13820 (A3772_13820)	comER	2620819..2621640 (+)	822	WP_004398597.1	late competence protein ComER	-
A3772_RS13825 (A3772_13825)	yqeM	2621706..2622449 (-)	744	WP_003229973.1	class I SAM-dependent DNA methyltransferase	-
A3772_RS13830 (A3772_13830)	rsfS	2622446..2622802 (-)	357	WP_003229971.1	ribosome silencing factor	-
A3772_RS13835 (A3772_13835)	yqeK	2622820..2623380 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
A3772_RS13840 (A3772_13840)	nadD	2623370..2623939 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
A3772_RS13845 (A3772_13845)	yhbY	2623951..2624241 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
A3772_RS13850 (A3772_13850)	aroE	2624235..2625077 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=176064 A3772_RS13815 WP_004398514.1 2620118..2620735(-) (comEA) [Bacillus subtilis strain SZMC 6179J isolate B23]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=176064 A3772_RS13815 WP_004398514.1 2620118..2620735(-) (comEA) [Bacillus subtilis strain SZMC 6179J isolate B23]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39