Detailed information NTG0175787

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: A2I97_RS12250
• Genome accession: NZ_CP014990
• Coordinates: 2522106..2522720 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain KD1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2517106..2527720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A2I97_RS12225 (A2I97_11840)	rpsT	2517406..2517672 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
A2I97_RS12230 (A2I97_11845)	holA	2517689..2518730 (-)	1042	Protein_2352	DNA polymerase III subunit delta	-
A2I97_RS19590	-	2518770..2518924 (-)	155	Protein_2353	hypothetical protein	-
A2I97_RS12235 (A2I97_19370)	-	2518965..2519099 (+)	135	WP_003152870.1	YqzM family protein	-
A2I97_RS12240 (A2I97_11850)	comEC	2519118..2521469 (-)	2352	WP_070082133.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A2I97_RS12245 (A2I97_11855)	-	2521470..2522039 (-)	570	WP_003152868.1	ComE operon protein 2	-
A2I97_RS12250 (A2I97_11860)	comEA	2522106..2522720 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
A2I97_RS12255 (A2I97_11865)	comER	2522778..2523599 (+)	822	WP_014305449.1	late competence protein ComER	-
A2I97_RS12260 (A2I97_11870)	-	2523668..2524405 (-)	738	WP_013352944.1	class I SAM-dependent DNA methyltransferase	-
A2I97_RS12265 (A2I97_11875)	rsfS	2524402..2524758 (-)	357	WP_003152864.1	ribosome silencing factor	-
A2I97_RS12270 (A2I97_11880)	yqeK	2524776..2525336 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
A2I97_RS12275 (A2I97_11885)	-	2525326..2525895 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
A2I97_RS12280 (A2I97_11890)	yhbY	2525906..2526196 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
A2I97_RS12285 (A2I97_11895)	aroE	2526190..2527032 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=175787 A2I97_RS12250 WP_013352942.1 2522106..2522720(-) (comEA) [Bacillus velezensis strain KD1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=175787 A2I97_RS12250 WP_013352942.1 2522106..2522720(-) (comEA) [Bacillus velezensis strain KD1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363