Detailed information NTG0174812

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: A3224_RS02770
• Genome accession: NZ_CP014864
• Coordinates: 650555..651964 (-)
• Length: 469 a.a.
• NCBI ID: WP_067151180.1
• Uniprot ID: A0A143HJF9
• Organism: Microbulbifer thermotolerans strain DAU221
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 645555..656964

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A3224_RS02755 (A3224_02755)	-	645658..649056 (+)	3399	WP_082817361.1	PilC/PilY family type IV pilus protein	-
A3224_RS02760 (A3224_02760)	-	649122..649562 (+)	441	WP_067151176.1	type IV pilin protein	-
A3224_RS02765 (A3224_02765)	-	649662..650231 (-)	570	WP_067151178.1	GspH/FimT family pseudopilin	-
A3224_RS02770 (A3224_02770)	pilR	650555..651964 (-)	1410	WP_067151180.1	sigma-54 dependent transcriptional regulator	Regulator
A3224_RS02775 (A3224_02775)	-	652087..653682 (-)	1596	WP_067151183.1	PAS domain-containing sensor histidine kinase	-
A3224_RS02780 (A3224_02780)	-	653774..655402 (+)	1629	WP_067151186.1	NAD+ synthase	-
A3224_RS02785 (A3224_02785)	-	655433..656377 (-)	945	WP_067151189.1	outer membrane protein assembly factor BamD	-

Sequence

Protein

• Length: 469 a.a.
• Molecular weight: 52304.69 Da
• Isoelectric Point: 6.4503

>NTDB_id=174812 A3224_RS02770 WP_067151180.1 650555..651964(-) (pilR) [Microbulbifer thermotolerans strain DAU221]
MEPLALVIDDEPDICELLALTLQRMDVHCHTAATLREAQRLLDENRYDFCLTDIRLPDGDGLQLVEHIQAQDKSDLPVAV
ITAHGNMDTAIKALKLGAFDFVSKPVDLERLRSLVQLALRLNHKRTDTQKPNSDHLLLGETEKMRELRRQIAKVARSDAP
IYISGESGSGKELAARSIHAQGPRADKPFVPINCGAIPAELMESEFFGHKKGSFTGAHRDKPGLMQAAEGGTLFLDEVAD
LPLDMQVKLLRAIQEKSVRPIGASQEVPIDVRILSATHKDLAKEVAEGRFRNDLYYRINVIEITVPPLRERKEDIPLLAQ
TIMQRIATNAGVSPPHLSAEAMEALKNYQFPGNVRQLENTLERAFTLSDQQIIRAQDLHLEREALSATRSAEQPTPPRPP
SQPAAAQTYPGQFDPSHYGSLDSFLQAIEKEAIEKALNETRWNRTAAAQKLGISFRSLRYRLKKLGLED

Nucleotide

• Length: 1410 bp

>NTDB_id=174812 A3224_RS02770 WP_067151180.1 650555..651964(-) (pilR) [Microbulbifer thermotolerans strain DAU221]
ATGGAACCCTTGGCCCTGGTCATCGACGATGAGCCCGATATCTGTGAGCTGCTAGCCCTGACATTGCAGCGCATGGACGT
TCACTGCCACACCGCCGCCACACTGAGGGAGGCGCAGCGGTTGTTGGATGAGAATCGCTATGATTTTTGCCTCACCGATA
TACGCCTGCCAGACGGCGACGGCCTACAGCTGGTGGAGCACATCCAAGCGCAAGATAAAAGCGATCTGCCGGTGGCGGTC
ATCACCGCACACGGGAATATGGATACCGCTATCAAGGCGCTCAAACTGGGCGCCTTTGATTTCGTCAGCAAACCGGTGGA
TCTGGAACGACTGCGTAGTCTGGTGCAATTGGCGCTGCGCCTGAACCACAAGCGCACTGATACGCAAAAACCAAATTCTG
ATCACCTATTGCTCGGCGAAACCGAAAAGATGCGGGAGTTGCGCCGCCAAATCGCCAAGGTGGCACGCAGCGATGCCCCT
ATCTATATCAGCGGAGAATCTGGCTCCGGCAAGGAATTGGCAGCCCGATCGATCCACGCCCAAGGGCCACGCGCCGACAA
ACCTTTTGTGCCGATCAACTGCGGCGCGATTCCCGCGGAATTGATGGAAAGCGAATTTTTTGGTCACAAAAAGGGCAGCT
TCACCGGCGCCCACAGAGACAAACCCGGTCTGATGCAGGCCGCCGAAGGCGGGACCCTCTTCCTGGATGAAGTGGCGGAC
CTCCCTTTAGACATGCAGGTTAAACTGCTGCGCGCGATACAGGAAAAATCCGTGCGCCCAATCGGCGCCAGTCAGGAGGT
ACCGATAGACGTCCGTATTCTCAGTGCTACCCACAAAGATCTCGCCAAGGAAGTGGCCGAAGGTCGCTTCCGCAACGATC
TCTACTACCGTATCAACGTTATTGAAATCACTGTGCCCCCACTGCGTGAGCGCAAAGAAGATATCCCTCTCCTGGCACAA
ACCATTATGCAGCGCATCGCCACCAATGCCGGTGTTTCCCCTCCCCACCTCTCTGCAGAGGCGATGGAAGCGTTAAAGAA
CTATCAATTCCCCGGCAACGTGCGCCAACTGGAAAACACCCTGGAGCGCGCCTTTACACTGAGCGACCAACAGATTATTC
GCGCACAGGATTTGCATTTGGAGCGGGAGGCGCTGTCAGCAACACGTAGCGCAGAACAACCGACTCCTCCCCGTCCCCCT
TCCCAGCCTGCCGCAGCCCAGACCTATCCCGGCCAGTTCGACCCAAGCCACTACGGCTCCCTGGACAGCTTCCTCCAGGC
AATTGAAAAAGAAGCCATCGAGAAAGCTTTGAACGAAACCCGCTGGAATAGAACCGCCGCAGCTCAAAAGCTTGGCATCA
GTTTTCGCTCCCTGCGGTACCGGCTGAAAAAATTGGGATTGGAAGATTAA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (6-115)

• Bacterial regulatory protein, Fis family (HTH_8) (425-465)

• Sigma-54 interaction domain (Sigma54_activat) (138-304)

3D structure

• AlphaFold DB: A0A143HJF9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	60.345	98.934	0.597
pilR	Acinetobacter baumannii strain A118	48.729	100	0.49
luxO	Vibrio cholerae strain A1552	37.037	97.868	0.362