Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   A3L20_RS22580 Genome accession   NZ_CP014847
Coordinates   4483484..4484230 (-) Length   248 a.a.
NCBI ID   WP_000487007.1    Uniprot ID   A0A9W3JRD4
Organism   Bacillus thuringiensis strain HD12     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4478484..4489230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3L20_RS22565 (A3L20_22565) dnaG 4479245..4481041 (-) 1797 WP_000528221.1 DNA primase -
  A3L20_RS35760 - 4481123..4481266 (-) 144 WP_000479749.1 hypothetical protein -
  A3L20_RS36590 - 4481403..4481534 (-) 132 WP_003259477.1 hypothetical protein -
  A3L20_RS22570 (A3L20_22570) - 4481902..4482714 (-) 813 WP_000368949.1 pyruvate, water dikinase regulatory protein -
  A3L20_RS22575 (A3L20_22575) - 4482744..4483376 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  A3L20_RS22580 (A3L20_22580) recO 4483484..4484230 (-) 747 WP_000487007.1 DNA repair protein RecO Machinery gene
  A3L20_RS22585 (A3L20_22585) - 4484264..4484407 (-) 144 WP_000883924.1 YqzL family protein -
  A3L20_RS22590 (A3L20_22590) era 4484584..4485489 (-) 906 WP_001080236.1 GTPase Era -
  A3L20_RS22595 (A3L20_22595) - 4485482..4485880 (-) 399 WP_001086287.1 cytidine deaminase -
  A3L20_RS22600 (A3L20_22600) - 4485980..4486333 (-) 354 WP_000715475.1 diacylglycerol kinase family protein -
  A3L20_RS22605 (A3L20_22605) ybeY 4486330..4486800 (-) 471 WP_000054680.1 rRNA maturation RNase YbeY -
  A3L20_RS22610 (A3L20_22610) - 4486797..4488941 (-) 2145 WP_000091515.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28622.20 Da        Isoelectric Point: 8.0566

>NTDB_id=174728 A3L20_RS22580 WP_000487007.1 4483484..4484230(-) (recO) [Bacillus thuringiensis strain HD12]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKISAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDSEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=174728 A3L20_RS22580 WP_000487007.1 4483484..4484230(-) (recO) [Bacillus thuringiensis strain HD12]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGATAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGGTTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTTATTCAAATGGGATCAGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATTCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTCAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment