Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A3L20_RS22320 Genome accession   NZ_CP014847
Coordinates   4430770..4431813 (-) Length   347 a.a.
NCBI ID   WP_001013183.1    Uniprot ID   A0A9X6VF82
Organism   Bacillus thuringiensis strain HD12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4425770..4436813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3L20_RS22275 (A3L20_22275) - 4426203..4426403 (-) 201 WP_000106083.1 YqzE family protein -
  A3L20_RS22280 (A3L20_22280) - 4426442..4426939 (-) 498 WP_000836665.1 shikimate kinase -
  A3L20_RS22285 (A3L20_22285) - 4427055..4427705 (-) 651 WP_000183866.1 2OG-Fe(II) oxygenase -
  A3L20_RS22290 (A3L20_22290) comGG 4427880..4428251 (-) 372 WP_042595685.1 competence type IV pilus minor pilin ComGG -
  A3L20_RS22295 (A3L20_22295) comGF 4428248..4428718 (-) 471 WP_000923110.1 competence type IV pilus minor pilin ComGF -
  A3L20_RS22300 (A3L20_22300) - 4428688..4428990 (-) 303 WP_000829465.1 hypothetical protein -
  A3L20_RS22305 (A3L20_22305) comGD 4428983..4429438 (-) 456 WP_002082168.1 competence type IV pilus minor pilin ComGD -
  A3L20_RS22310 (A3L20_22310) comGC 4429435..4429734 (-) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  A3L20_RS22315 (A3L20_22315) comGB 4429746..4430783 (-) 1038 WP_025989085.1 competence type IV pilus assembly protein ComGB -
  A3L20_RS22320 (A3L20_22320) comGA 4430770..4431813 (-) 1044 WP_001013183.1 competence type IV pilus ATPase ComGA Machinery gene
  A3L20_RS22325 (A3L20_22325) - 4432019..4432714 (+) 696 WP_000434110.1 helix-turn-helix transcriptional regulator -
  A3L20_RS22330 (A3L20_22330) - 4432841..4433083 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  A3L20_RS22335 (A3L20_22335) - 4433191..4434585 (+) 1395 WP_016077255.1 L-cystine transporter -
  A3L20_RS36245 (A3L20_22340) - 4434665..4435056 (-) 392 Protein_4468 hypothetical protein -
  A3L20_RS22345 (A3L20_22345) - 4435144..4435359 (-) 216 WP_001008325.1 DUF3912 family protein -
  A3L20_RS22350 (A3L20_22350) - 4435613..4435924 (+) 312 WP_001093238.1 hypothetical protein -
  A3L20_RS22355 (A3L20_22355) - 4435959..4436447 (-) 489 WP_000764268.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39196.60 Da        Isoelectric Point: 8.8958

>NTDB_id=174727 A3L20_RS22320 WP_001013183.1 4430770..4431813(-) (comGA) [Bacillus thuringiensis strain HD12]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LRIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=174727 A3L20_RS22320 WP_001013183.1 4430770..4431813(-) (comGA) [Bacillus thuringiensis strain HD12]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCGAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment