Detailed information NTG0174560

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BCBMB205_RS12220
• Genome accession: NZ_CP014838
• Coordinates: 2518478..2519092 (-)
• Length: 204 a.a.
• NCBI ID: WP_032873955.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CBMB205
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2513478..2524092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCBMB205_RS12195 (BCBMB205_24760)	rpsT	2513781..2514047 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BCBMB205_RS12200 (BCBMB205_24770)	holA	2514064..2515105 (-)	1042	Protein_2338	DNA polymerase III subunit delta	-
BCBMB205_RS19725	-	2515145..2515296 (-)	152	Protein_2339	hypothetical protein	-
BCBMB205_RS12205 (BCBMB205_24780)	-	2515337..2515471 (+)	135	WP_003152870.1	YqzM family protein	-
BCBMB205_RS12210 (BCBMB205_24790)	comEC	2515490..2517841 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BCBMB205_RS12215 (BCBMB205_24800)	-	2517842..2518411 (-)	570	WP_003152868.1	ComE operon protein 2	-
BCBMB205_RS12220 (BCBMB205_24810)	comEA	2518478..2519092 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
BCBMB205_RS12225 (BCBMB205_24820)	comER	2519151..2519972 (+)	822	WP_012118027.1	late competence protein ComER	-
BCBMB205_RS12230 (BCBMB205_24830)	-	2520041..2520778 (-)	738	WP_032873951.1	class I SAM-dependent DNA methyltransferase	-
BCBMB205_RS12235 (BCBMB205_24840)	rsfS	2520775..2521131 (-)	357	WP_007408260.1	ribosome silencing factor	-
BCBMB205_RS12240 (BCBMB205_24850)	yqeK	2521135..2521710 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BCBMB205_RS12245 (BCBMB205_24860)	-	2521700..2522269 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BCBMB205_RS12250 (BCBMB205_24870)	yhbY	2522280..2522570 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BCBMB205_RS12255 (BCBMB205_24880)	aroE	2522564..2523406 (-)	843	WP_032873946.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22265.47 Da
• Isoelectric Point: 6.4940

>NTDB_id=174560 BCBMB205_RS12220 WP_032873955.1 2518478..2519092(-) (comEA) [Bacillus velezensis strain CBMB205]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=174560 BCBMB205_RS12220 WP_032873955.1 2518478..2519092(-) (comEA) [Bacillus velezensis strain CBMB205]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363