Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   BFL21_RS22950 Genome accession   NZ_CP018243
Coordinates   4144853..4145485 (+) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli strain 350     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 4139853..4150485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL21_RS22920 (BFL21_22715) yheS 4140022..4141935 (+) 1914 WP_000634798.1 ABC transporter ATP-binding protein -
  BFL21_RS22925 (BFL21_22720) yheT 4141935..4142957 (+) 1023 WP_000057377.1 hydrolase -
  BFL21_RS22930 (BFL21_22725) yheU 4142951..4143169 (+) 219 WP_000907085.1 YheU family protein -
  BFL21_RS22935 (BFL21_22730) prkB 4143223..4144092 (+) 870 WP_001274677.1 phosphoribulokinase -
  BFL21_RS22940 (BFL21_22735) yhfA 4144147..4144551 (-) 405 WP_001148908.1 OsmC family protein -
  BFL21_RS22950 (BFL21_22745) crp 4144853..4145485 (+) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  BFL21_RS22955 (BFL21_22750) yhfK 4145536..4147626 (+) 2091 WP_001295162.1 YccS/YhfK family putative transporter -
  BFL21_RS22960 (BFL21_22755) argD 4147693..4148913 (-) 1221 WP_000963819.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  BFL21_RS22965 (BFL21_22760) pabA 4148999..4149562 (-) 564 WP_000601867.1 aminodeoxychorismate synthase component 2 -
  BFL21_RS22970 (BFL21_22765) fic 4149594..4150196 (-) 603 WP_001280650.1 putative adenosine monophosphate-protein transferase Fic -
  BFL21_RS22975 (BFL21_22770) yhfG 4150186..4150353 (-) 168 WP_000736862.1 YhfG family protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=174409 BFL21_RS22950 WP_000242755.1 4144853..4145485(+) (crp) [Escherichia coli strain 350]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=174409 BFL21_RS22950 WP_000242755.1 4144853..4145485(+) (crp) [Escherichia coli strain 350]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCTGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTCAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTTGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment