Detailed information NTG0173985

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: A1R12_RS12130
• Genome accession: NZ_CP014783
• Coordinates: 2563203..2563817 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain B15
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2558203..2568817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1R12_RS12110 (A1R12_12110)	rpsT	2558506..2558772 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
A1R12_RS12115 (A1R12_12115)	holA	2558789..2559830 (-)	1042	Protein_2412	DNA polymerase III subunit delta	-
A1R12_RS20120	-	2559870..2560021 (-)	152	Protein_2413	hypothetical protein	-
A1R12_RS19750	-	2560062..2560196 (+)	135	WP_003152870.1	YqzM family protein	-
A1R12_RS12120 (A1R12_12120)	comEC	2560215..2562566 (-)	2352	WP_061890729.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A1R12_RS12125 (A1R12_12125)	-	2562567..2563136 (-)	570	WP_003152868.1	ComE operon protein 2	-
A1R12_RS12130 (A1R12_12130)	comEA	2563203..2563817 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
A1R12_RS12135 (A1R12_12135)	comER	2563876..2564697 (+)	822	WP_039254409.1	late competence protein ComER	-
A1R12_RS12140 (A1R12_12140)	-	2564766..2565503 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
A1R12_RS12145 (A1R12_12145)	rsfS	2565500..2565856 (-)	357	WP_007408260.1	ribosome silencing factor	-
A1R12_RS12150 (A1R12_12150)	yqeK	2565874..2566434 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
A1R12_RS12155 (A1R12_12155)	-	2566424..2566993 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
A1R12_RS12160 (A1R12_12160)	yhbY	2567004..2567294 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
A1R12_RS12165 (A1R12_12165)	aroE	2567288..2568130 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=173985 A1R12_RS12130 WP_015240245.1 2563203..2563817(-) (comEA) [Bacillus amyloliquefaciens strain B15]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=173985 A1R12_RS12130 WP_015240245.1 2563203..2563817(-) (comEA) [Bacillus amyloliquefaciens strain B15]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363