Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ASQ50_RS04705 Genome accession   NZ_CP014754
Coordinates   1006762..1007637 (+) Length   291 a.a.
NCBI ID   WP_058089900.1    Uniprot ID   A0A142FNH4
Organism   Marinobacter sp. LQ44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1001762..1012637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASQ50_RS04690 (ASQ50_04685) - 1002913..1003362 (-) 450 WP_058089903.1 pilin -
  ASQ50_RS04695 (ASQ50_04690) pilB 1003753..1005465 (+) 1713 WP_058089902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ASQ50_RS04700 (ASQ50_04695) pilC 1005468..1006688 (+) 1221 WP_058089901.1 type II secretion system F family protein Machinery gene
  ASQ50_RS04705 (ASQ50_04700) pilD 1006762..1007637 (+) 876 WP_058089900.1 prepilin peptidase Machinery gene
  ASQ50_RS04710 (ASQ50_04705) coaE 1007735..1008340 (+) 606 WP_058089899.1 dephospho-CoA kinase -
  ASQ50_RS04715 (ASQ50_04710) - 1008423..1009421 (+) 999 WP_227510106.1 hypothetical protein -
  ASQ50_RS04720 (ASQ50_04715) tsaA 1009423..1010172 (+) 750 WP_058089897.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ASQ50_RS04725 (ASQ50_04720) - 1010216..1012570 (+) 2355 WP_058089896.1 hybrid sensor histidine kinase/response regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32183.51 Da        Isoelectric Point: 7.5112

>NTDB_id=173623 ASQ50_RS04705 WP_058089900.1 1006762..1007637(+) (pilD) [Marinobacter sp. LQ44]
MPSLDQLLSTPWLLYLTVTLFSLCIGSFLNVVILRLPKMMQQEWRCQCEEFLEVPEKQRKQEAPLSLSKPASTCPSCGHK
IRAWENIPVISWLVLRGKCSSCKAPISPRYPIIEAITAIFSVVTIALLGPTEAALWALLLVWALVALTMIDFDTQLLPDS
ITLPLMWLGLVLNYFGVLTDFNSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSL
VGAVVGIALMVFKKHGREVPIPFGPYLAAAGLLCLWFGVEIQAIWFGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=173623 ASQ50_RS04705 WP_058089900.1 1006762..1007637(+) (pilD) [Marinobacter sp. LQ44]
ATGCCCTCTCTAGACCAACTCCTCTCCACCCCCTGGCTCCTATACCTCACCGTCACCCTGTTCTCCCTCTGCATCGGCAG
CTTCCTGAACGTAGTCATCCTGCGCCTGCCCAAAATGATGCAGCAGGAATGGCGTTGCCAGTGTGAAGAGTTTCTGGAGG
TACCGGAAAAGCAAAGAAAGCAGGAAGCACCACTTTCCCTTTCAAAGCCTGCGTCTACCTGCCCCTCCTGTGGCCATAAG
ATTCGGGCCTGGGAAAATATCCCGGTGATCAGCTGGCTGGTGCTGCGGGGCAAATGTTCGTCTTGTAAAGCGCCTATTTC
GCCGCGCTACCCAATCATCGAAGCCATTACCGCCATCTTCTCGGTAGTAACCATCGCTTTGCTGGGGCCAACTGAAGCGG
CGCTTTGGGCCCTGCTGCTGGTATGGGCCCTGGTGGCGCTGACGATGATCGATTTCGACACACAACTGCTGCCAGACAGC
ATCACCCTGCCCCTGATGTGGCTCGGCCTGGTGTTGAACTATTTTGGCGTGTTGACGGACTTTAACAGTGCCTTCTGGGG
TGCCGTTGCCGGGTACCTGTCGCTTTGGTCGGTGTATTGGTTGTTCAAGCTGGTGACTGGCAAAGAAGGTATGGGGCATG
GGGATTTCAAGCTGTTGGCGGCTCTGGGTGCCTGGTTGGGGTGGCAGCTGCTGCCGGCGGTGATTTTGCTGTCGTCGCTG
GTGGGGGCGGTGGTTGGTATTGCGCTGATGGTGTTCAAAAAGCATGGGCGCGAGGTGCCAATTCCGTTTGGGCCTTATCT
GGCGGCGGCTGGGTTGCTGTGTTTGTGGTTTGGGGTGGAGATTCAGGCGATTTGGTTTGGGTTTTTGGGGGTTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A142FNH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.839

94.158

0.526
  pilD Acinetobacter nosocomialis M2

55.474

94.158

0.522
  pilD Vibrio campbellii strain DS40M4

53.237

95.533

0.509
  pilD Vibrio cholerae strain A1552

54.212

93.814

0.509
  pilD Neisseria gonorrhoeae MS11

49.206

86.598

0.426

Multiple sequence alignment