Detailed information NTG0173399

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AS588_RS03515
• Genome accession: NZ_CP014700
• Coordinates: 703417..704031 (+)
• Length: 204 a.a.
• NCBI ID: WP_015387983.1
• Uniprot ID: A0A6A8LHQ3
• Organism: Bacillus amyloliquefaciens strain S499
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 698417..709031

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS588_RS03480 (AS588_03480)	aroE	699104..699946 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
AS588_RS03485 (AS588_03485)	yhbY	699940..700230 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AS588_RS03490 (AS588_03490)	-	700241..700810 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
AS588_RS03495 (AS588_03495)	yqeK	700800..701381 (+)	582	WP_015387982.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AS588_RS03500 (AS588_03500)	rsfS	701378..701734 (+)	357	WP_014305451.1	ribosome silencing factor	-
AS588_RS03505 (AS588_03505)	-	701731..702468 (+)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
AS588_RS03510 (AS588_03510)	comER	702537..703358 (-)	822	WP_003152866.1	late competence protein ComER	-
AS588_RS03515 (AS588_03515)	comEA	703417..704031 (+)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
AS588_RS03520 (AS588_03520)	-	704098..704667 (+)	570	WP_003152868.1	ComE operon protein 2	-
AS588_RS03525 (AS588_03525)	comEC	704668..707019 (+)	2352	WP_015387984.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AS588_RS19235	-	707038..707172 (-)	135	WP_003152870.1	YqzM family protein	-
AS588_RS19800	-	707213..707367 (+)	155	Protein_693	hypothetical protein	-
AS588_RS03530 (AS588_03530)	holA	707407..708448 (+)	1042	Protein_694	DNA polymerase III subunit delta	-
AS588_RS03535 (AS588_03535)	rpsT	708465..708731 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22255.41 Da
• Isoelectric Point: 5.5211

>NTDB_id=173399 AS588_RS03515 WP_015387983.1 703417..704031(+) (comEA) [Bacillus amyloliquefaciens strain S499]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=173399 AS588_RS03515 WP_015387983.1 703417..704031(+) (comEA) [Bacillus amyloliquefaciens strain S499]
ATGACGAAAGCAATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A6A8LHQ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363