Detailed information NTG0172355

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AYR70_RS14300
• Genome accession: NZ_CP014541
• Coordinates: 3008911..3010029 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain XH856
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3003911..3015029

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AYR70_RS14290 (AYR70_14290)	-	3007027..3007719 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
AYR70_RS14295 (AYR70_14295)	pilT	3007846..3008883 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AYR70_RS14300 (AYR70_14300)	pilU	3008911..3010029 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AYR70_RS14305 (AYR70_14305)	fur	3010099..3010536 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AYR70_RS14310 (AYR70_14310)	-	3010648..3011046 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AYR70_RS14315 (AYR70_14315)	-	3011101..3011424 (-)	324	WP_000442587.1	RnfH family protein	-
AYR70_RS14320 (AYR70_14320)	-	3011424..3012491 (-)	1068	WP_002019572.1	hypothetical protein	-
AYR70_RS14330 (AYR70_14330)	-	3012644..3013099 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AYR70_RS14335 (AYR70_14335)	-	3013352..3014200 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=172355 AYR70_RS14300 WP_000347039.1 3008911..3010029(+) (pilU) [Acinetobacter baumannii strain XH856]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=172355 AYR70_RS14300 WP_000347039.1 3008911..3010029(+) (pilU) [Acinetobacter baumannii strain XH856]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395