Detailed information NTG0171738

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AWV81_RS13305
• Genome accession: NZ_CP014471
• Coordinates: 2458648..2459265 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. natto strain CGMCC 2108
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2453648..2464265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AWV81_RS13280 (AWV81_13275)	rpsT	2453950..2454216 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
AWV81_RS13285 (AWV81_13280)	holA	2454231..2455274 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
AWV81_RS23105	-	2455314..2455463 (-)	150	WP_003229985.1	hypothetical protein	-
AWV81_RS13290 (AWV81_13285)	yqzM	2455504..2455638 (+)	135	WP_003229983.1	YqzM family protein	-
AWV81_RS13295 (AWV81_13290)	comEC	2455678..2458008 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AWV81_RS13300 (AWV81_13295)	comEB	2458012..2458581 (-)	570	WP_003229978.1	ComE operon protein 2	-
AWV81_RS13305 (AWV81_13300)	comEA	2458648..2459265 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
AWV81_RS13310 (AWV81_13305)	comER	2459349..2460170 (+)	822	WP_014480313.1	late competence protein ComER	-
AWV81_RS13315 (AWV81_13310)	yqeM	2460236..2460979 (-)	744	WP_014480314.1	class I SAM-dependent methyltransferase	-
AWV81_RS13320 (AWV81_13315)	rsfS	2460976..2461332 (-)	357	WP_014480315.1	ribosome silencing factor	-
AWV81_RS13325 (AWV81_13320)	yqeK	2461350..2461910 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AWV81_RS13330 (AWV81_13325)	nadD	2461900..2462469 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AWV81_RS13335 (AWV81_13330)	yhbY	2462481..2462771 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AWV81_RS13340 (AWV81_13335)	aroE	2462765..2463607 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=171738 AWV81_RS13305 WP_014480312.1 2458648..2459265(-) (comEA) [Bacillus subtilis subsp. natto strain CGMCC 2108]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=171738 AWV81_RS13305 WP_014480312.1 2458648..2459265(-) (comEA) [Bacillus subtilis subsp. natto strain CGMCC 2108]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39