Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AYM25_RS08490 Genome accession   NZ_CP014407
Coordinates   1657500..1657997 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain USA300-SUR12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1652500..1662997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYM25_RS08465 (AYM25_08225) gcvPB 1652662..1654134 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  AYM25_RS08470 (AYM25_08230) gcvPA 1654127..1655473 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AYM25_RS08475 (AYM25_08235) gcvT 1655493..1656584 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AYM25_RS08480 (AYM25_08240) - 1656743..1657267 (-) 525 WP_001015117.1 shikimate kinase -
  AYM25_RS08485 (AYM25_08245) - 1657257..1657403 (-) 147 WP_001789879.1 hypothetical protein -
  AYM25_RS08490 (AYM25_08250) comGF 1657500..1657997 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  AYM25_RS08495 (AYM25_08255) comGE 1657915..1658214 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AYM25_RS08500 (AYM25_08260) comGD 1658201..1658647 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  AYM25_RS08505 (AYM25_08265) comGC 1658625..1658936 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AYM25_RS08510 (AYM25_08270) comGB 1658950..1660020 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AYM25_RS08515 (AYM25_08275) comGA 1659992..1660966 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AYM25_RS08520 (AYM25_08280) - 1661018..1661641 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AYM25_RS08525 (AYM25_08285) - 1661638..1661967 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AYM25_RS08530 (AYM25_08290) - 1661967..1662953 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=171150 AYM25_RS08490 WP_001789864.1 1657500..1657997(-) (comGF) [Staphylococcus aureus strain USA300-SUR12]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=171150 AYM25_RS08490 WP_001789864.1 1657500..1657997(-) (comGF) [Staphylococcus aureus strain USA300-SUR12]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment