Detailed information NTG0170648

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AYM39_RS00720
• Genome accession: NZ_CP014360
• Coordinates: 165702..166856 (-)
• Length: 384 a.a.
• NCBI ID: WP_054759524.1
• Uniprot ID: A0A172U3G9
• Organism: Methylomonas sp. DH-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 160702..171856

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AYM39_RS00700 (AYM39_00695)	-	161226..161948 (+)	723	WP_054759463.1	cytochrome c1	-
AYM39_RS00705 (AYM39_00700)	ftsH	162034..163947 (-)	1914	WP_082880752.1	ATP-dependent zinc metalloprotease FtsH	-
AYM39_RS00710 (AYM39_00705)	lexA	164519..165148 (+)	630	WP_064023307.1	transcriptional repressor LexA	-
AYM39_RS23540	-	165161..165310 (+)	150	WP_157198033.1	hypothetical protein	-
AYM39_RS00715 (AYM39_00710)	-	165356..165679 (+)	324	WP_054759467.1	hypothetical protein	-
AYM39_RS00720 (AYM39_00715)	pilU	165702..166856 (-)	1155	WP_054759524.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AYM39_RS00725 (AYM39_00720)	-	167018..169627 (+)	2610	WP_064020086.1	PAS domain-containing protein	-
AYM39_RS00730 (AYM39_00725)	-	169624..170256 (+)	633	WP_064020087.1	response regulator transcription factor	-
AYM39_RS00735 (AYM39_00730)	-	170425..171468 (+)	1044	WP_064020088.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
AYM39_RS00740 (AYM39_00735)	-	171465..171689 (+)	225	WP_064020089.1	sulfurtransferase TusA family protein	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 42483.63 Da
• Isoelectric Point: 6.0981

>NTDB_id=170648 AYM39_RS00720 WP_054759524.1 165702..166856(-) (pilU) [Methylomonas sp. DH-1]
MEFRDYLKILVQHDGSDLYLTAGAPPAAKFQGTLKPLENVKLTPERIRQIADSVMDNEQRASFEHVPEMNLAITEEGIGR
FRVNIFKQRNCYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKRSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFVSQRLVPTIEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENVGMQTFDSHL
LKLYKSGVISLEEAMRNSDSPNNLKLKINLSEGLGSATKPAGALGSLALEEISKKEGEDGDEGH

Nucleotide

• Length: 1155 bp

>NTDB_id=170648 AYM39_RS00720 WP_054759524.1 165702..166856(-) (pilU) [Methylomonas sp. DH-1]
ATGGAATTTAGAGACTATTTGAAAATCCTGGTGCAGCACGACGGTTCCGATTTGTATTTGACGGCCGGCGCGCCGCCGGC
TGCAAAATTTCAAGGCACCTTGAAGCCGCTGGAAAACGTCAAGCTGACTCCGGAGCGGATCAGGCAGATTGCCGACAGCG
TGATGGACAACGAGCAGCGCGCCAGTTTCGAACACGTACCGGAGATGAATCTGGCGATTACCGAAGAGGGCATCGGCCGA
TTCCGGGTCAATATTTTCAAGCAACGCAATTGTTATGCGTTGGTGATCCGCAATATCAAGGTGGATATTCCGAATGCCGA
CGTTTTAGGCTTGCCGCAAATTTTGAAAGACAAAATCATGGAAAAGCGCGGCCTGATTTTGTTCGTCGGCGGCACCGGTT
CCGGCAAATCGACCTCGCTGGCCGCCTTGATCGATTACCGCAACAGCAATTCGTCGGGCCACATCATCACCATCGAAGAC
CCGATCGAATTCGTTCACCCGCATAAGCGTTCGCTGGTCAACCAGCGCGAAGTCGGCGTCGACACGCTGAGTTACGAGGA
CGCATTGAAAAACACCTTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACGATGGAACATG
CGTTAGCCTTTGCCGAAACCGGACATTTGTGCCTGTCGACCTTGCATGCCAATAACGCCAACCAGGCGCTGGATAGGATC
ATCAATTTCTTCCCGGAAGAGCGCCGGCCGCAGTTGTTGATGGACTTGTCGTTGAATTTGCAGGCCTTCGTTTCGCAACG
TCTGGTACCGACCATCGAAGGCAAGCGGGTTGCGGCAATCGAAATCCTGTTGGGCACAAAATTGGTCAGCGATTTGATCC
ATAAAGGCGACATTCACGCGATCAAGGAGGCGATGGAAAAGTCGGAAAACGTCGGTATGCAGACCTTCGACAGCCATCTA
TTGAAGCTGTACAAAAGCGGCGTGATCTCACTGGAAGAAGCGATGCGCAATTCCGATTCGCCGAACAATCTGAAATTGAA
AATCAACTTGAGCGAGGGCTTGGGTTCGGCGACCAAACCGGCCGGGGCGCTGGGCAGTCTGGCTTTGGAAGAAATCAGCA
AGAAGGAAGGCGAGGACGGCGACGAAGGCCATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-269)

3D structure

• AlphaFold DB: A0A172U3G9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.12	91.406	0.531
pilU	Acinetobacter baylyi ADP1	54.696	94.271	0.516
pilU	Vibrio cholerae strain A1552	49.435	92.188	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.052	100	0.404
pilT	Legionella pneumophila strain Lp02	42.424	85.938	0.365
pilT	Legionella pneumophila strain ERS1305867	42.424	85.938	0.365