Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AXW78_RS20225 Genome accession   NZ_CP014282
Coordinates   4051204..4052247 (-) Length   347 a.a.
NCBI ID   WP_061884576.1    Uniprot ID   A0A9W3YJ60
Organism   Bacillus thuringiensis strain Bt185     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4046204..4057247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW78_RS20180 (AXW78_20180) - 4046638..4046838 (-) 201 WP_000106083.1 YqzE family protein -
  AXW78_RS20185 (AXW78_20185) - 4046877..4047374 (-) 498 WP_000836648.1 shikimate kinase -
  AXW78_RS20190 (AXW78_20190) - 4047489..4048139 (-) 651 WP_000183865.1 2OG-Fe(II) oxygenase -
  AXW78_RS20195 (AXW78_20195) comGG 4048314..4048685 (-) 372 WP_001231319.1 competence type IV pilus minor pilin ComGG -
  AXW78_RS20200 (AXW78_20200) comGF 4048682..4049152 (-) 471 WP_061884575.1 competence type IV pilus minor pilin ComGF -
  AXW78_RS20205 (AXW78_20205) - 4049122..4049424 (-) 303 WP_000829463.1 hypothetical protein -
  AXW78_RS20210 (AXW78_20210) comGD 4049417..4049872 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  AXW78_RS20215 (AXW78_20215) comGC 4049869..4050168 (-) 300 WP_001178685.1 competence type IV pilus major pilin ComGC -
  AXW78_RS20220 (AXW78_20220) comGB 4050180..4051217 (-) 1038 WP_002164182.1 competence type IV pilus assembly protein ComGB -
  AXW78_RS20225 (AXW78_20225) comGA 4051204..4052247 (-) 1044 WP_061884576.1 competence type IV pilus ATPase ComGA Machinery gene
  AXW78_RS20230 (AXW78_20230) - 4052453..4053148 (+) 696 WP_000434104.1 helix-turn-helix domain-containing protein -
  AXW78_RS20235 (AXW78_20235) - 4053274..4053516 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  AXW78_RS20240 (AXW78_20240) - 4053623..4055017 (+) 1395 WP_001094346.1 L-cystine transporter -
  AXW78_RS20245 (AXW78_20245) - 4055117..4055488 (-) 372 WP_000875396.1 hypothetical protein -
  AXW78_RS20250 (AXW78_20250) - 4055576..4055791 (-) 216 WP_001008332.1 DUF3912 family protein -
  AXW78_RS20255 (AXW78_20255) - 4056044..4056355 (+) 312 WP_001093237.1 hypothetical protein -
  AXW78_RS20260 (AXW78_20260) - 4056389..4056877 (-) 489 WP_000764269.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39144.52 Da        Isoelectric Point: 8.5399

>NTDB_id=170200 AXW78_RS20225 WP_061884576.1 4051204..4052247(-) (comGA) [Bacillus thuringiensis strain Bt185]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSQLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=170200 AXW78_RS20225 WP_061884576.1 4051204..4052247(-) (comGA) [Bacillus thuringiensis strain Bt185]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCCTTATCACAACTTTCGTTATTTCCAAGTACAGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATACGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTGGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment