Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BIZ52_RS06265 Genome accession   NZ_CP017457
Coordinates   1387894..1389378 (-) Length   494 a.a.
NCBI ID   WP_027226834.1    Uniprot ID   I7HZ91
Organism   Legionella pneumophila subsp. fraseri strain Detroit-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1382894..1394378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ52_RS06250 (BIZ52_06175) cysS 1383884..1385254 (+) 1371 WP_027226831.1 cysteine--tRNA ligase -
  BIZ52_RS06255 (BIZ52_06180) - 1385435..1387348 (+) 1914 WP_027226832.1 DUF3413 domain-containing protein -
  BIZ52_RS06260 (BIZ52_06185) - 1387478..1387792 (+) 315 WP_027226833.1 DUF6482 family protein -
  BIZ52_RS06265 (BIZ52_06190) pilB 1387894..1389378 (-) 1485 WP_027226834.1 GspE family T2SS ATPase variant LspE Machinery gene
  BIZ52_RS06270 (BIZ52_06195) lspD 1389382..1391757 (-) 2376 WP_027226835.1 GspD family T2SS secretin variant LspD -
  BIZ52_RS06275 (BIZ52_06200) - 1391929..1392957 (+) 1029 WP_027226836.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55122.47 Da        Isoelectric Point: 5.9033

>NTDB_id=169034 BIZ52_RS06265 WP_027226834.1 1387894..1389378(-) (pilB) [Legionella pneumophila subsp. fraseri strain Detroit-1]
MENEEKSLKIPYGFAKSNGIVVGEIKENLAVVYHLPNTSLQAFAEIKRLLQCELDLRKVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSLLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPKHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGIGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHQLREEE
KELMGLNPDTDVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENLQTMENYLRPSTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=169034 BIZ52_RS06265 WP_027226834.1 1387894..1389378(-) (pilB) [Legionella pneumophila subsp. fraseri strain Detroit-1]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACGGCTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATCAAAGA
AAATCTGGCTGTTGTCTATCATTTACCTAACACATCTCTACAAGCTTTCGCTGAGATAAAACGATTATTGCAATGTGAAC
TTGACTTAAGAAAAGTCGATGAATCTACTTTTCAGCAGCATTTGGCTAATATTTACCAATCCAAATCCTCTATACTAGAT
GCTGCAGAAGGCATGGAAGAAGACATGGATTTGTCCTTGTTGGCCAGTCAACTACCTGTCAGTGAGGATTTATTGGAAAA
TCAGGATGATGCCCCAATTATCCGATTACTCAATGCTCTATTCACTCAAGCCATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTAGTGCGTAACCGAATTGACGGAGTTCTACAAGAAGTCCTTGAAATTCAACGAGCT
ATCGCACCTTTGGTCATTTCCAGAGTCAAGGTGATGGCCAAGTTGGATATAGCAGAAAAGCGAATTCCCCAAGATGGGCG
AATTTCATTGCGCATAGGAGGGCACAATATTGATGTCAGGGTCTCAACTTTACCATCAAATCATGGAGAACGAGTGGTTT
TAAGGATATTGGATAAGCAAGCCGCTCAATTGGATTTAAATCTTTTGGGTATGCCTAAACATACACTAAAAACAGTACGA
CAAATGATTGCAGAACCTCATGGCATCATTTTAGTCACAGGCCCCACAGGTTCAGGCAAAACAACCTCTCTCTATGCCAT
GCTTACTGAATTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACTTACCTGGTATAGGAC
AGACGCAAGTTAATACTAAAGTTCAAATGACTTTCGCCAAAGGATTAAGAGCTATATTACGTCAAGATCCGGATGTTGTA
ATGATAGGAGAGATCCGTGATTTGGAAACAGCTGAAATCGCAGTTCAAGCCAGCCTAACCGGACATCTGGTACTATCAAC
CCTTCATACCAATACTGCATTGGGTGCGCTAACCCGATTACATGATATGGGGGTTGAGTCCTTTTTATTGTCATCCAGTA
TAGTAGGCCTCATCGCGCAACGCTTGGTAAGAAAATTATGTTCTCATTGTAAAACACCTCATCAATTAAGAGAGGAAGAA
AAAGAGCTAATGGGTCTTAATCCGGATACTGATGTCTCACAAGTCTTTGAACCTAAGGGATGTGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACCGGAATTTATGAATTAATTATCGTAGATGAAACTCTCCGTGGAATGATCCATCGTAATGAAA
ATCTTCAAACCATGGAAAATTATTTACGGCCATCTACACCAAGTATACGAGAAGATGGTTTTAAAAGAGTATTATGTGGA
GACACCTCACTTGCAGAAATTTTAAGGGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7HZ91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.319

93.927

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

42.094

90.891

0.383
  pilB Acinetobacter baumannii D1279779

45.433

84.211

0.383
  pilB Vibrio parahaemolyticus RIMD 2210633

46.305

82.186

0.381
  pilB Acinetobacter baylyi ADP1

48.072

78.745

0.379
  pilB Vibrio campbellii strain DS40M4

46.059

82.186

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36