Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL552_RS14440 Genome accession   NZ_CP014094
Coordinates   2730572..2731588 (-) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio diabolicus strain FDAARGOS_96     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2725572..2736588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL552_RS14415 (AL552_22570) aroK 2726436..2726954 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AL552_RS14420 (AL552_22575) pilQ 2727142..2728890 (-) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  AL552_RS14425 (AL552_22580) pilP 2728922..2729437 (-) 516 WP_012841352.1 pilus assembly protein PilP Machinery gene
  AL552_RS14430 (AL552_22585) pilO 2729427..2730020 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL552_RS14435 (AL552_22590) pilN 2730013..2730588 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  AL552_RS14440 (AL552_22595) pilM 2730572..2731588 (-) 1017 WP_005395718.1 type IV pilus biogenesis protein PilM Machinery gene
  AL552_RS14445 (AL552_22600) - 2731731..2734292 (+) 2562 WP_104969861.1 penicillin-binding protein 1A -
  AL552_RS14450 (AL552_22605) oxyR 2734344..2735255 (-) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  AL552_RS14455 (AL552_22610) - 2735427..2736155 (+) 729 WP_104969862.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=168343 AL552_RS14440 WP_005395718.1 2730572..2731588(-) (pilM) [Vibrio diabolicus strain FDAARGOS_96]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=168343 AL552_RS14440 WP_005395718.1 2730572..2731588(-) (pilM) [Vibrio diabolicus strain FDAARGOS_96]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAACTGCAAATAGAACAAAACCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCAGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACACGCGGAGCAAACAGCCCTGTTCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment