Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AL519_RS04225 Genome accession   NZ_CP014064
Coordinates   779826..780323 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain FDAARGOS_159     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774826..785323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL519_RS04200 (AL519_04145) gcvPB 774988..776460 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  AL519_RS04205 (AL519_04150) gcvPA 776453..777799 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AL519_RS04210 (AL519_04155) gcvT 777819..778910 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AL519_RS04215 (AL519_04160) - 779069..779593 (-) 525 WP_031882602.1 shikimate kinase -
  AL519_RS04220 (AL519_04165) - 779583..779729 (-) 147 WP_001792109.1 hypothetical protein -
  AL519_RS04225 (AL519_04170) comGF 779826..780323 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  AL519_RS04230 (AL519_04175) comGE 780241..780540 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  AL519_RS04235 (AL519_04180) comGD 780527..780973 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  AL519_RS04240 (AL519_04185) comGC 780951..781262 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AL519_RS04245 (AL519_04190) comGB 781276..782346 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  AL519_RS04250 (AL519_04195) comGA 782318..783292 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  AL519_RS04255 (AL519_04200) - 783344..783968 (-) 625 Protein_771 MBL fold metallo-hydrolase -
  AL519_RS04260 (AL519_04205) - 783965..784294 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AL519_RS04265 (AL519_04210) - 784294..785280 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=168229 AL519_RS04225 WP_001788897.1 779826..780323(-) (comGF) [Staphylococcus aureus strain FDAARGOS_159]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=168229 AL519_RS04225 WP_001788897.1 779826..780323(-) (comGF) [Staphylococcus aureus strain FDAARGOS_159]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment