Detailed information NTG0168018

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AL549_RS18920
• Genome accession: NZ_CP014049
• Coordinates: 2260513..2261382 (+)
• Length: 289 a.a.
• NCBI ID: WP_061058910.1
• Uniprot ID: B7U4S3
• Organism: Vibrio vulnificus strain FDAARGOS_119
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2255513..2266382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AL549_RS18900 (AL549_17385)	nadC	2255897..2256784 (+)	888	WP_061058907.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AL549_RS18905 (AL549_17390)	-	2257007..2257456 (+)	450	WP_175546417.1	pilin	-
AL549_RS18910 (AL549_17395)	pilB	2257461..2259146 (+)	1686	WP_061058908.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AL549_RS18915 (AL549_17400)	pilC	2259193..2260419 (+)	1227	WP_061058909.1	type II secretion system F family protein	Machinery gene
AL549_RS18920 (AL549_17405)	pilD	2260513..2261382 (+)	870	WP_061058910.1	prepilin peptidase	Machinery gene
AL549_RS18925 (AL549_17410)	coaE	2261384..2261992 (+)	609	WP_061058911.1	dephospho-CoA kinase	-
AL549_RS18930 (AL549_17415)	zapD	2262025..2262765 (+)	741	WP_011079546.1	cell division protein ZapD	-
AL549_RS18935 (AL549_17420)	yacG	2262828..2263022 (+)	195	WP_026060801.1	DNA gyrase inhibitor YacG	-
AL549_RS18940 (AL549_17425)	rplS	2263247..2263600 (-)	354	WP_011079544.1	50S ribosomal protein L19	-
AL549_RS18945 (AL549_17430)	trmD	2263642..2264391 (-)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
AL549_RS18950 (AL549_17435)	rimM	2264419..2264967 (-)	549	WP_061058912.1	ribosome maturation factor RimM	-
AL549_RS18955 (AL549_17440)	rpsP	2264983..2265231 (-)	249	WP_011079541.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32277.29 Da
• Isoelectric Point: 6.4630

>NTDB_id=168018 AL549_RS18920 WP_061058910.1 2260513..2261382(+) (pilD) [Vibrio vulnificus strain FDAARGOS_119]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLISATFIDLDTMLLPDQLTLP
LTWAGIALALLEISPVSLQDSVIGTMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Nucleotide

• Length: 870 bp

>NTDB_id=168018 AL549_RS18920 WP_061058910.1 2260513..2261382(+) (pilD) [Vibrio vulnificus strain FDAARGOS_119]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATAATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGCTCAAGTTGTCCTTCGTGCCATACCCCAATACGAGTTCGA
GATAATATTCCTGTCTTTAGTTGGTTAGCACTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGCTATCC
TTTTGTTGAAGCACTGAGCGCCTTTTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCACTGA
TTGGTTTTACTTTGGTGTTGATCTCCGCCACCTTCATCGACTTAGATACCATGTTACTGCCTGATCAGCTGACACTGCCG
CTGACGTGGGCAGGCATCGCGCTAGCGTTACTAGAGATAAGCCCAGTATCTCTGCAGGATTCAGTGATCGGTACGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCCGCTTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTCTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAACAAGGCATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTGATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATAGGGGCATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: B7U4S3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	76.736	99.654	0.765
pilD	Vibrio cholerae strain A1552	71.181	99.654	0.709
pilD	Acinetobacter nosocomialis M2	53.986	95.502	0.516
pilD	Acinetobacter baumannii D1279779	53.986	95.502	0.516
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457