Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL537_RS02770 Genome accession   NZ_CP014036
Coordinates   564528..565544 (-) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio diabolicus strain FDAARGOS_105     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559528..570544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL537_RS02745 (AL537_02745) aroK 560398..560916 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AL537_RS02750 (AL537_02750) pilQ 561104..562852 (-) 1749 WP_006742777.1 type IV pilus secretin PilQ family protein Machinery gene
  AL537_RS02755 (AL537_02755) pilP 562878..563393 (-) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  AL537_RS02760 (AL537_02760) pilO 563383..563976 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL537_RS02765 (AL537_02765) pilN 563969..564544 (-) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  AL537_RS02770 (AL537_02770) pilM 564528..565544 (-) 1017 WP_005395718.1 type IV pilus assembly protein PilM Machinery gene
  AL537_RS02775 (AL537_02775) - 565688..568249 (+) 2562 WP_048626702.1 PBP1A family penicillin-binding protein -
  AL537_RS02780 (AL537_02780) oxyR 568301..569212 (-) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  AL537_RS02790 (AL537_02790) - 569383..570111 (+) 729 WP_005395721.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=167737 AL537_RS02770 WP_005395718.1 564528..565544(-) (pilM) [Vibrio diabolicus strain FDAARGOS_105]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=167737 AL537_RS02770 WP_005395718.1 564528..565544(-) (pilM) [Vibrio diabolicus strain FDAARGOS_105]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAATTGCAAATAGAACAAAACCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACACGCGGAGCAAACAGCCCTGTTCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment