NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	BHT24_RS17285	Genome accession	NZ_CP017220
Coordinates	3314211..3314870 (+)	Length	219 a.a.
NCBI ID	WP_001221502.1	Uniprot ID	B7LGG8
Organism	Escherichia coli strain FAM21845
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3309211..3319870

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BHT24_RS17245 (BHT24_16555)	ygiV	3309549..3310004 (-)	456	WP_244557681.1	AraC family transcriptional regulator	-
BHT24_RS27535	-	3310065..3310804 (+)	740	Protein_3189	IS3-like element IS629 family transposase	-
BHT24_RS17270 (BHT24_16575)	ltrA	3311477..3312985 (+)	1509	WP_001189111.1	group II intron reverse transcriptase/maturase	-
BHT24_RS17275 (BHT24_16580)	-	3313069..3313548 (+)	480	Protein_3191	IS3 family transposase	-
BHT24_RS17280 (BHT24_16585)	ygiW	3313667..3314059 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BHT24_RS17285 (BHT24_16590)	ciaR	3314211..3314870 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
BHT24_RS17290 (BHT24_16595)	qseC	3314867..3316216 (+)	1350	WP_000673354.1	quorum sensing histidine kinase QseC	-
BHT24_RS17295 (BHT24_16600)	ygiZ	3316262..3316594 (-)	333	WP_000914691.1	DUF2645 family protein	-
BHT24_RS28365 (BHT24_16605)	-	3316601..3317311 (-)	711	WP_000834029.1	hypothetical protein	-
BHT24_RS28370 (BHT24_16610)	-	3317314..3317793 (-)	480	WP_000065333.1	Hcp family type VI secretion system effector	-
BHT24_RS17310 (BHT24_16615)	mdaB	3318043..3318624 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BHT24_RS17315 (BHT24_16620)	ygiN	3318655..3318969 (+)	315	WP_000958598.1	putative quinol monooxygenase	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24745.66 Da Isoelectric Point: 6.6543

                                >NTDB_id=167066 BHT24_RS17285 WP_001221502.1 3314211..3314870(+) (ciaR) [Escherichia coli strain FAM21845]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA

RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=167066 BHT24_RS17285 WP_001221502.1 3314211..3314870(+) (ciaR) [Escherichia coli strain FAM21845]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG

CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365

Detailed information NTG0167066