Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AWQ23_RS03635 Genome accession   NZ_CP013998
Coordinates   757379..759400 (+) Length   673 a.a.
NCBI ID   WP_062432634.1    Uniprot ID   -
Organism   Picosynechococcus sp. PCC 73109     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 752379..764400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ23_RS03615 (AWQ23_03615) - 752648..752902 (-) 255 WP_062432624.1 sulfurtransferase TusA family protein -
  AWQ23_RS03620 (AWQ23_03620) dnaJ 752899..754026 (-) 1128 WP_062432627.1 molecular chaperone DnaJ -
  AWQ23_RS03625 (AWQ23_03625) dnaK 754148..756103 (-) 1956 WP_030005934.1 molecular chaperone DnaK -
  AWQ23_RS03630 (AWQ23_03630) grpE 756220..756969 (-) 750 WP_062432630.1 nucleotide exchange factor GrpE -
  AWQ23_RS03635 (AWQ23_03635) pilB/pilB1 757379..759400 (+) 2022 WP_062432634.1 GspE/PulE family protein Machinery gene
  AWQ23_RS03640 (AWQ23_03640) pilT 759521..760627 (+) 1107 WP_012306326.1 type IV pilus twitching motility protein PilT Machinery gene
  AWQ23_RS03645 (AWQ23_03645) - 760694..761932 (+) 1239 WP_062432637.1 type II secretion system F family protein -
  AWQ23_RS03650 (AWQ23_03650) - 761943..762500 (+) 558 WP_062432640.1 hypothetical protein -
  AWQ23_RS03655 (AWQ23_03655) - 762497..763573 (-) 1077 WP_062432656.1 glycosyl transferase -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75448.43 Da        Isoelectric Point: 5.1820

>NTDB_id=167056 AWQ23_RS03635 WP_062432634.1 757379..759400(+) (pilB/pilB1) [Picosynechococcus sp. PCC 73109]
MTHSSLPKRTRALALRNDFSPFGNKLIQQGVVDHQQMEKALVETRRSGRPLTAIVEELTGKALSPELVRQYKKHHLFELK
ILYGLNSIDPEVQKIDIDQMGELINSFVPVELCRRHRLLPIRRVQDEVIVAMVEPDNLAAQDDLNRLFRLQNLTLRRIVI
TEDDYRQVLDGYLEQQVQIQIQQQEQAAKAAEAAELRKAVDIEDLDLGVAGELDEQEEESADLMNENQASSAPVIKLVNK
VLLKALQEGASDIHIEPQENDLRIRFRKDGVLQYAFDPLPKRIVPAVTARFKIMADLDIAERRSPQDGRIRRVFKGRKVD
FRVNSLPSRYGEKIVLRILDNSSTQLGLDLLITNQDILGQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNEPGINI
STAEDPIEYTLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIA
RLDEMGVEPFMISGSLLGVLAQRLMRRVCGECRIPYQPTVEELARYGLSSNDRHPLTVYRANTLTPEQIQMAKEAGSLCP
KCGGGGYKGRVGVYEFMQNSEEIERLINTGATTDMIKEVAVQEGMLSILAYSLELVKEGHTTFEEVDRVTFTDSGLEAEL
KAKRKTGLVCDTCNAELQPDWLDCPYCMTPRFS

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=167056 AWQ23_RS03635 WP_062432634.1 757379..759400(+) (pilB/pilB1) [Picosynechococcus sp. PCC 73109]
ATGACCCACTCTTCCCTTCCCAAACGGACAAGAGCCCTTGCCCTCCGCAATGATTTCTCTCCTTTCGGCAACAAGCTCAT
TCAACAGGGAGTTGTAGACCATCAACAGATGGAAAAAGCCCTCGTTGAAACCCGGCGTAGTGGACGCCCCCTCACGGCCA
TTGTCGAAGAACTAACCGGGAAAGCCCTGTCCCCTGAGCTAGTCCGACAGTACAAAAAGCATCATCTCTTTGAACTGAAG
ATCCTCTACGGTCTCAATTCCATTGACCCAGAAGTTCAAAAAATTGACATCGATCAGATGGGCGAGTTGATCAATTCCTT
CGTGCCCGTAGAATTATGTCGCCGCCATCGTCTCCTCCCGATCCGGCGGGTTCAAGATGAAGTGATTGTGGCGATGGTAG
AGCCCGATAACCTAGCCGCCCAAGATGATTTAAACCGTCTTTTTCGCCTGCAAAATCTGACGTTACGGCGCATCGTGATC
ACAGAAGATGACTATCGTCAGGTTCTAGATGGCTATTTAGAACAGCAAGTCCAGATTCAAATCCAACAGCAGGAACAAGC
AGCCAAGGCCGCCGAAGCAGCGGAACTGAGAAAAGCAGTTGATATCGAAGATCTTGATCTCGGTGTAGCCGGGGAATTAG
ACGAGCAGGAAGAAGAAAGCGCCGACCTGATGAATGAAAATCAGGCTAGCTCTGCTCCGGTGATCAAACTCGTCAATAAA
GTGCTGCTCAAAGCCCTCCAGGAAGGGGCCTCAGATATTCACATCGAACCCCAAGAAAATGATCTGCGGATTCGTTTCCG
TAAAGATGGGGTTTTGCAATATGCTTTTGATCCGCTGCCCAAACGGATTGTGCCAGCAGTGACGGCCCGGTTCAAAATTA
TGGCGGACCTAGACATTGCCGAACGTCGTTCGCCTCAGGATGGTCGGATTCGGCGGGTGTTTAAGGGGCGCAAGGTGGAC
TTTCGGGTCAATAGCCTACCCAGTCGCTACGGCGAAAAAATTGTTTTACGGATTCTCGATAATTCTTCAACCCAGTTGGG
CTTGGATTTGTTAATTACCAACCAAGATATTCTGGGGCAGGTGCGGGAAATGGCCCAGCGGCCATTTGGTCTAATTTTGG
TGACGGGGCCAACAGGCTCAGGAAAATCAACAACTTTGTATTCAATTCTGGCGGAGCGCAACGAGCCAGGCATCAACATC
AGTACCGCAGAAGACCCCATTGAATATACTTTGCCGGGCATTACCCAGGTGCAGGTCATCCGGGAGAAGGGGATGGATTT
TGCCTCAATTTTGCGGGCATTTTTGCGGCAGGATCCAGATGTCATTCTTGTGGGGGAAACCCGGGATAAGGAAACGGCAA
AAACAGCCATTGAGGCGGCTTTAACGGGGCACTTGGTACTAACGACTCTCCACACCAATGATGCTCCAGGGGCGATCGCC
CGTCTAGATGAGATGGGAGTTGAACCCTTTATGATTTCCGGGTCACTCCTGGGAGTTCTGGCCCAGCGCTTGATGCGACG
GGTTTGTGGGGAATGCCGCATCCCCTATCAGCCCACAGTGGAAGAGCTGGCACGCTACGGCCTTTCCAGTAATGATCGCC
ATCCTTTAACGGTGTACCGGGCCAATACCCTGACGCCAGAGCAAATCCAAATGGCCAAAGAAGCCGGCAGTCTCTGTCCT
AAATGTGGCGGCGGCGGCTACAAAGGCCGGGTGGGGGTCTATGAGTTTATGCAAAATAGCGAAGAAATTGAACGGTTGAT
TAATACCGGGGCCACCACGGACATGATCAAAGAAGTGGCAGTGCAAGAGGGCATGTTGAGTATCTTGGCCTACAGTTTGG
AGTTGGTCAAAGAAGGCCATACGACCTTTGAGGAAGTAGACCGTGTTACCTTTACGGACTCTGGTCTTGAGGCAGAACTA
AAGGCGAAACGAAAAACGGGCCTGGTTTGTGACACCTGTAATGCTGAGTTGCAGCCGGATTGGCTAGATTGTCCCTACTG
TATGACGCCCCGCTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(240-507)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.68

100

0.66

Multiple sequence alignment