Detailed information NTG0166933

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: APT60_RS23955
• Genome accession: NZ_CP013989
• Coordinates: 5207157..5208494 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5202157..5213494

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APT60_RS23930 (APT60_23930)	pgeF	5202300..5203028 (-)	729	WP_003102588.1	peptidoglycan editing factor PgeF	-
APT60_RS23935 (APT60_23935)	rluD	5203025..5203987 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
APT60_RS23940 (APT60_23940)	-	5204133..5205158 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
APT60_RS23945 (APT60_23945)	-	5205315..5205560 (+)	246	WP_009316269.1	PP0621 family protein	-
APT60_RS23950 (APT60_23950)	pilS	5205550..5207142 (+)	1593	WP_003123394.1	two-component system sensor histidine kinase PilS	Regulator
APT60_RS23955 (APT60_23955)	pilR	5207157..5208494 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
APT60_RS23960 (APT60_23960)	thiO	5208540..5209634 (-)	1095	WP_020750388.1	glycine oxidase ThiO	-
APT60_RS23965 (APT60_23965)	-	5209769..5210278 (+)	510	WP_003102595.1	GspH/FimT family pseudopilin	-
APT60_RS23970 (APT60_23970)	-	5210384..5210890 (+)	507	WP_003102598.1	GspH/FimT family pseudopilin	-
APT60_RS23975 (APT60_23975)	pilV	5210881..5211438 (+)	558	WP_003119423.1	type 4a pilus minor pilin PilV	Machinery gene
APT60_RS23980 (APT60_23980)	pilW	5211435..5212259 (+)	825	WP_003119422.1	type 4a pilus minor pilin PilW	-
APT60_RS23985 (APT60_23985)	pilX	5212256..5212843 (+)	588	WP_030048355.1	type 4a pilus minor pilin PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=166933 APT60_RS23955 WP_003094694.1 5207157..5208494(+) (pilR) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=166933 APT60_RS23955 WP_003094694.1 5207157..5208494(+) (pilR) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384