Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BS16045_RS13750 Genome accession   NZ_CP017112
Coordinates   2611831..2612448 (-) Length   205 a.a.
NCBI ID   WP_032722140.1    Uniprot ID   -
Organism   Bacillus subtilis strain BS16045     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606831..2617448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS16045_RS13725 (BS16045_02707) rpsT 2607134..2607400 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  BS16045_RS13730 holA 2607415..2608457 (-) 1043 Protein_2646 DNA polymerase III subunit delta -
  BS16045_RS22405 - 2608497..2608646 (-) 150 WP_120363468.1 hypothetical protein -
  BS16045_RS13735 (BS16045_02710) yqzM 2608687..2608821 (+) 135 WP_003229983.1 YqzM family protein -
  BS16045_RS13740 (BS16045_02711) comEC 2608861..2611191 (-) 2331 WP_069322764.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BS16045_RS13745 (BS16045_02712) comEB 2611195..2611764 (-) 570 WP_003229978.1 ComE operon protein 2 -
  BS16045_RS13750 (BS16045_02713) comEA 2611831..2612448 (-) 618 WP_032722140.1 competence protein ComEA Machinery gene
  BS16045_RS13755 (BS16045_02714) comER 2612532..2613353 (+) 822 WP_015714289.1 late competence protein ComER -
  BS16045_RS13760 (BS16045_02715) yqeM 2613419..2614162 (-) 744 WP_069322765.1 class I SAM-dependent DNA methyltransferase -
  BS16045_RS13765 (BS16045_02716) rsfS 2614159..2614515 (-) 357 WP_041333759.1 ribosome silencing factor -
  BS16045_RS13770 (BS16045_02717) yqeK 2614533..2615093 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BS16045_RS13775 (BS16045_02718) nadD 2615083..2615652 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  BS16045_RS13780 (BS16045_02719) yhbY 2615664..2615954 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BS16045_RS13785 (BS16045_02720) aroE 2615948..2616790 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21748.46 Da        Isoelectric Point: 4.8354

>NTDB_id=166609 BS16045_RS13750 WP_032722140.1 2611831..2612448(-) (comEA) [Bacillus subtilis strain BS16045]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=166609 BS16045_RS13750 WP_032722140.1 2611831..2612448(-) (comEA) [Bacillus subtilis strain BS16045]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38