Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   NEB5A_RS18655 Genome accession   NZ_CP017100
Coordinates   3752437..3753105 (-) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli strain K-12 NEB 5-alpha     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3747437..3758105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEB5A_RS18625 (NEB5A_18625) rfaQ 3747610..3748680 (-) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -
  NEB5A_RS18630 (NEB5A_18630) waaA 3749086..3750363 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  NEB5A_RS18635 (NEB5A_18635) coaD 3750371..3750850 (+) 480 WP_046613194.1 pantetheine-phosphate adenylyltransferase -
  NEB5A_RS18640 (NEB5A_18640) mutM 3750889..3751698 (-) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  NEB5A_RS18645 (NEB5A_18645) rpmG 3751796..3751963 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  NEB5A_RS18650 (NEB5A_18650) rpmB 3751984..3752220 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  NEB5A_RS18655 (NEB5A_18655) radC 3752437..3753105 (-) 669 WP_001350561.1 DNA repair protein RadC Machinery gene
  NEB5A_RS18660 (NEB5A_18660) coaBC 3753277..3754497 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  NEB5A_RS18665 (NEB5A_18665) dut 3754475..3754933 (+) 459 WP_000976073.1 dUTP diphosphatase -
  NEB5A_RS18670 (NEB5A_18670) slmA 3755040..3755636 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  NEB5A_RS18675 (NEB5A_18675) pyrE 3755673..3756314 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  NEB5A_RS18680 (NEB5A_18680) rph 3756380..3757096 (-) 717 WP_001247096.1 ribonuclease PH -
  NEB5A_RS18685 (NEB5A_18685) yicC 3757223..3758086 (+) 864 WP_000621340.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=166485 NEB5A_RS18655 WP_001350561.1 3752437..3753105(-) (radC) [Escherichia coli strain K-12 NEB 5-alpha]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=166485 NEB5A_RS18655 WP_001350561.1 3752437..3753105(-) (radC) [Escherichia coli strain K-12 NEB 5-alpha]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment