Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   BFV99_RS32060 Genome accession   NZ_CP016955
Coordinates   6601684..6602328 (+) Length   214 a.a.
NCBI ID   WP_003085214.1    Uniprot ID   A0A0H2ZLY6
Organism   Pseudomonas aeruginosa strain RIVM-EMC2982     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 6596684..6607328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFV99_RS32035 (BFV99_31030) - 6597176..6598657 (-) 1482 WP_003101642.1 AAA family ATPase -
  BFV99_RS32040 (BFV99_31035) - 6598736..6599074 (+) 339 WP_003085225.1 histidine triad nucleotide-binding protein -
  BFV99_RS32045 (BFV99_31040) coq7 6599174..6599821 (+) 648 WP_003085224.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  BFV99_RS32050 (BFV99_31045) speD 6599876..6600670 (-) 795 WP_003101641.1 adenosylmethionine decarboxylase -
  BFV99_RS32055 (BFV99_31050) - 6600990..6601412 (-) 423 WP_003085219.1 OsmC family protein -
  BFV99_RS32060 (BFV99_31055) crp 6601684..6602328 (+) 645 WP_003085214.1 cAMP-activated global transcriptional regulator CRP Regulator
  BFV99_RS32065 (BFV99_31060) trpC 6602390..6603226 (-) 837 WP_003085205.1 indole-3-glycerol phosphate synthase TrpC -
  BFV99_RS32070 (BFV99_31065) trpD 6603223..6604272 (-) 1050 WP_003085203.1 anthranilate phosphoribosyltransferase -
  BFV99_RS32075 (BFV99_31070) - 6604274..6604879 (-) 606 WP_003101640.1 aminodeoxychorismate/anthranilate synthase component II -
  BFV99_RS32080 (BFV99_31075) - 6605227..6605484 (-) 258 WP_003118919.1 hypothetical protein -
  BFV99_RS32085 (BFV99_31080) - 6605481..6605843 (-) 363 WP_003101639.1 hypothetical protein -
  BFV99_RS32090 (BFV99_31085) - 6605840..6606469 (-) 630 WP_003085188.1 glycoside hydrolase family 19 protein -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24225.01 Da        Isoelectric Point: 8.0525

>NTDB_id=165544 BFV99_RS32060 WP_003085214.1 6601684..6602328(+) (crp) [Pseudomonas aeruginosa strain RIVM-EMC2982]
MVAITHTPKLKHLDKLLAHCHRRRYTAKSTIIYAGDRCETLFFIIKGSVTILIEDDDGREMIIGYLNSGDFFGELGLFEK
EGSEQERSAWVRAKVECEVAEISYAKFRELSQQDSEILYTLGSQMADRLRKTTRKVGDLAFLDVTGRVARTLLDLCQQPD
AMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKSLEEQGLVHVKGKTMVVFGTR

Nucleotide


Download         Length: 645 bp        

>NTDB_id=165544 BFV99_RS32060 WP_003085214.1 6601684..6602328(+) (crp) [Pseudomonas aeruginosa strain RIVM-EMC2982]
ATGGTAGCTATTACCCACACACCCAAACTCAAACACCTAGACAAGCTGCTCGCACACTGTCACCGCCGCCGCTACACCGC
AAAGAGCACCATCATCTATGCCGGCGATCGCTGCGAAACGCTGTTCTTCATCATCAAGGGTTCGGTCACCATCCTCATCG
AGGACGACGACGGCCGCGAAATGATCATCGGCTACCTCAACAGCGGTGATTTCTTCGGCGAGCTGGGATTGTTCGAAAAG
GAAGGCAGCGAACAGGAACGCAGCGCCTGGGTTCGCGCCAAGGTGGAATGCGAAGTCGCCGAGATCAGCTATGCGAAGTT
CCGCGAACTGTCGCAGCAGGACTCGGAGATCCTCTACACCCTCGGCAGCCAGATGGCCGACCGCCTGCGCAAGACCACCC
GCAAGGTGGGCGACCTGGCCTTCCTCGACGTCACCGGACGCGTCGCCCGCACCTTGCTGGACCTGTGCCAGCAACCGGAC
GCCATGACCCACCCGGACGGCATGCAGATCAAGATCACCCGCCAGGAGATCGGCCGGATCGTCGGCTGCTCGCGGGAAAT
GGTCGGACGCGTGCTGAAGAGCCTGGAGGAGCAGGGCCTGGTGCATGTGAAAGGAAAGACCATGGTGGTCTTCGGCACCC
GCTGA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(22-114)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(146-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZLY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

67.327

94.393

0.636
  crp Haemophilus influenzae Rd KW20

60.396

94.393

0.57
  crp Acinetobacter baumannii D1279779

54.545

92.523

0.505

Multiple sequence alignment