Detailed information NTG0165307

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: BARD7_RS08065
• Genome accession: NZ_CP016913
• Coordinates: 1629748..1632333 (+)
• Length: 861 a.a.
• NCBI ID: WP_065982878.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain RD7-7
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1624748..1637333

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BARD7_RS08045 (BARD7_01647)	-	1625549..1626727 (+)	1179	WP_155759168.1	glycine C-acetyltransferase	-
BARD7_RS08050 (BARD7_01648)	miaB	1626870..1628399 (+)	1530	WP_065981488.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
BARD7_RS08055 (BARD7_01649)	ymcA	1628402..1628833 (+)	432	WP_065981489.1	RicAFT regulatory complex protein RicA family protein	Regulator
BARD7_RS08060 (BARD7_01650)	cotE	1629087..1629632 (+)	546	WP_065981490.1	outer spore coat protein CotE	-
BARD7_RS08065 (BARD7_01651)	hexA	1629748..1632333 (+)	2586	WP_065982878.1	DNA mismatch repair protein MutS	Machinery gene
BARD7_RS08070 (BARD7_01652)	hexB	1632349..1634226 (+)	1878	WP_065981491.1	DNA mismatch repair endonuclease MutL	Machinery gene
BARD7_RS08075 (BARD7_01653)	-	1634278..1635414 (-)	1137	WP_231131103.1	site-specific integrase	-
BARD7_RS08080	-	1635508..1636332 (+)	825	Protein_1578	DNA adenine methylase	-
BARD7_RS08085 (BARD7_01655)	-	1636447..1636965 (-)	519	WP_065981493.1	hypothetical protein	-

Sequence

Protein

• Length: 861 a.a.
• Molecular weight: 97527.89 Da
• Isoelectric Point: 5.4868

>NTDB_id=165307 BARD7_RS08065 WP_065982878.1 1629748..1632333(+) (hexA) [Bacillus amyloliquefaciens strain RD7-7]
MASYTPMIQQYLKIKAEYQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSAFPGGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNENTAAQLKERCGATISIEDGKMTERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERASLIDPCGDLLDLLEDALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGESREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLISRAQEILKQLEQSGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSHKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide

• Length: 2586 bp

>NTDB_id=165307 BARD7_RS08065 WP_065982878.1 1629748..1632333(+) (hexA) [Bacillus amyloliquefaciens strain RD7-7]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAATACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGGGACG
GAGGATCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAACAGACCGAAGATCCGAAGGCGGCCAAAGGCGTTGTCAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGACGGAAAAGGCATTCATGAGTCGGAAAACAATTTTATCGCTTCCGTTTCCGCATTTC
CTGGCGGATACGGCCTCGCTTTATCAGACTTAACGACTGGAGAAAACCTGGCCGTATTTATTGAACGGATTGAAGATGTC
ATGTCAGAAATTTATTCGGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAATGAAAATACAGCGGCCCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCCATCGAAGACGGGAAAATGACAGAACGGATTGAAATCGCAAAGCACTTGCCTGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGATCTGTATTCAAAACGGAATCTTGAACTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGATCACTGCTCTGGCTTTTGGACGAGACGAAAACAGCAATGGGCGGCAGGCTCCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCGTCCCAAATCGAAGAACGTCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAACGTGAAGATCTGCGCGAACGCCTGAAGGAAGTGTATGATTTAGAGCGTCTTGCGGGCCGGGTCGCATTCGGAAACGT
CAATGCAAGGGATTTAATCCAGCTGAAAGAATCGTTAAAACAGGTGCCGAGCATTAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCAGCTTGATTGACCCCTGCGGAGATCTGCTTGACCTGTTAGAAGATGCGCTGCATGAA
AACCCGCCGTTATCTTTAAAAGAAGGCAACTTAATAAAAGACGGCTATCATCAGAAACTCGATGAGTACCGTGACGCCAG
CAAAAACGGCAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCGTATACAGGCATCCGTTCATTAAAGGTCGGCT
TTAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTCCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACGCTCACAAACGCTGAACGGTACATTACGCCGGAATTAAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATTTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTTAAGCAATTTATTCCGCGCCTGCAGCGTCTAG
CCAAGCAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCAACGATCAGTGAAAACAGGCATTACACAAAGCCTGTCTTT
TCCGATAATGAGGTAAAAGTCATTGAAGGCAGACATCCTGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAATTGCCTGATGGGCGAATCACGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTTTATCAATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTTACGAGAATCGGGGCTGCGGACGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTAAACGCCACAAAAGACAGCCTGATCCTGTTCGATGAAATCGGACGCGGGACATCAACGTATGACGGAATGG
CGCTGGCACAGGCAATAATTGAATATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCACTATCATGAACTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCCGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCCGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGGTGATCTGA
TCAGCCGCGCGCAGGAGATTCTGAAACAGCTTGAGCAGTCAGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAGGAAGAACCGGCACAGCTGTCCTTTTTTGCAGACGGTGAAAAAGAACAGAAAGCACCGGCTGTTTCTCA
TAAAGAAAAACAAGTGCTTGAAGCATTTAAGTCGATCAATATTCTTGATATGACGCCTCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

• MutS domain II (MutS_II) (125-239)

• MutS domain V (MutS_V) (598-785)

• MutS domain I (MutS_I) (5-116)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	48.308	99.535	0.481
mutS	Pseudomonas stutzeri strain ATCC 17587	38.629	100	0.393