Detailed information NTG0164896

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FORC18_RS02965
• Genome accession: NZ_CP013826
• Coordinates: 586712..587581 (-)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus strain FORC_018
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 581712..592581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC18_RS02930 (FORC18_0480)	rpsP	582677..582925 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
FORC18_RS02935 (FORC18_0481)	rimM	582954..583502 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
FORC18_RS02940 (FORC18_0482)	trmD	583530..584273 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
FORC18_RS02945 (FORC18_0483)	rplS	584315..584668 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
FORC18_RS02950 (FORC18_0484)	yacG	585066..585260 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
FORC18_RS02955 (FORC18_0485)	zapD	585329..586069 (-)	741	WP_023624252.1	cell division protein ZapD	-
FORC18_RS02960 (FORC18_0486)	coaE	586097..586711 (-)	615	WP_025633754.1	dephospho-CoA kinase	-
FORC18_RS02965 (FORC18_0487)	pilD	586712..587581 (-)	870	WP_005493974.1	prepilin peptidase	Machinery gene
FORC18_RS02970 (FORC18_0488)	pilC	587646..588869 (-)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
FORC18_RS02975 (FORC18_0489)	pilB	588893..590578 (-)	1686	WP_020835398.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FORC18_RS02980 (FORC18_0490)	pilA	590578..590982 (-)	405	WP_015297250.1	pilin	Machinery gene
FORC18_RS02985 (FORC18_0491)	nadC	591246..592133 (-)	888	WP_025442237.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=164896 FORC18_RS02965 WP_005493974.1 586712..587581(-) (pilD) [Vibrio parahaemolyticus strain FORC_018]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=164896 FORC18_RS02965 WP_005493974.1 586712..587581(-) (pilD) [Vibrio parahaemolyticus strain FORC_018]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTTGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTGGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTGAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCAGTAATCAGCTGGCTATTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGCTATCC
GCTGATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCCGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45