Detailed information NTG0159855

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: A9256_RS01325
• Genome accession: NZ_CP016391
• Coordinates: 230403..230864 (+)
• Length: 153 a.a.
• NCBI ID: WP_001874812.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain FWL1402
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 225403..235864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9256_RS01295 (A9256_01295)	-	227018..227383 (+)	366	WP_000285373.1	DUF1033 family protein	-
A9256_RS01300 (A9256_01300)	comGA	227556..228527 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
A9256_RS01305 (A9256_01305)	comGB	228421..229464 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
A9256_RS01310 (A9256_01310)	comGC/cglC	229461..229790 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
A9256_RS01315 (A9256_01315)	comGD	229765..230178 (+)	414	WP_000244261.1	competence type IV pilus minor pilin ComGD	Machinery gene
A9256_RS01320 (A9256_01320)	comGE	230150..230449 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	Machinery gene
A9256_RS01325 (A9256_01325)	comGF	230403..230864 (+)	462	WP_001874812.1	competence type IV pilus minor pilin ComGF	Machinery gene
A9256_RS01330 (A9256_01330)	comGG	230842..231213 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
A9256_RS01335 (A9256_01335)	comGH	231328..232302 (+)	975	WP_001008574.1	class I SAM-dependent methyltransferase	Machinery gene
A9256_RS01340 (A9256_01340)	-	232334..233527 (+)	1194	WP_000047534.1	acetate kinase	-
A9256_RS01345 (A9256_01345)	-	233679..233885 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
A9256_RS10665	-	233944..234081 (+)	138	WP_001865900.1	hypothetical protein	-
A9256_RS01350 (A9256_01350)	-	234122..234577 (+)	456	WP_000905673.1	hypothetical protein	-
A9256_RS01355 (A9256_01355)	-	234646..235315 (+)	670	Protein_233	lysostaphin resistance A-like protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17875.89 Da
• Isoelectric Point: 9.8392

>NTDB_id=159855 A9256_RS01325 WP_001874812.1 230403..230864(+) (comGF) [Streptococcus agalactiae strain FWL1402]
MKIIRKFCTYQKLRVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 462 bp

>NTDB_id=159855 A9256_RS01325 WP_001874812.1 230403..230864(+) (comGF) [Streptococcus agalactiae strain FWL1402]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAGAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (49-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	47.887	92.81	0.444
comGF	Streptococcus mutans UA140	47.887	92.81	0.444
comGF/cglF	Streptococcus pneumoniae Rx1	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae D39	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae R6	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae TIGR4	43.165	90.85	0.392
comGF/cglF	Streptococcus mitis SK321	44.03	87.582	0.386
comGF/cglF	Streptococcus mitis NCTC 12261	43.284	87.582	0.379
comGF	Lactococcus lactis subsp. cremoris KW2	40.714	91.503	0.373