Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BA058_RS08915 Genome accession   NZ_CP016358
Coordinates   1854129..1854788 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain K-15KW01     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1849129..1859788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA058_RS08900 (BA058_08900) ygiS 1851334..1852941 (-) 1608 WP_001298384.1 ABC transporter substrate-binding protein -
  BA058_RS08905 (BA058_08905) ygiV 1853050..1853532 (-) 483 WP_000183491.1 GyrI-like domain-containing protein -
  BA058_RS08910 (BA058_08910) ygiW 1853585..1853977 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BA058_RS08915 (BA058_08915) ciaR 1854129..1854788 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  BA058_RS08920 (BA058_08920) qseC 1854785..1856134 (+) 1350 WP_000673389.1 quorum sensing histidine kinase QseC -
  BA058_RS08925 (BA058_08925) ygiZ 1856180..1856512 (-) 333 WP_000914696.1 DUF2645 family protein -
  BA058_RS28580 (BA058_08930) - 1856519..1857229 (-) 711 WP_000834021.1 hypothetical protein -
  BA058_RS28585 (BA058_08935) - 1857232..1857711 (-) 480 WP_000065328.1 Hcp family type VI secretion system effector -
  BA058_RS08940 (BA058_08940) mdaB 1857961..1858542 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BA058_RS08945 (BA058_08945) ygiN 1858573..1858887 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=159780 BA058_RS08915 WP_001221493.1 1854129..1854788(+) (ciaR) [Escherichia coli strain K-15KW01]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=159780 BA058_RS08915 WP_001221493.1 1854129..1854788(+) (ciaR) [Escherichia coli strain K-15KW01]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment