Detailed information NTG0159544

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: WL1483_RS16645
• Genome accession: NZ_CP013067
• Coordinates: 3476155..3477024 (+)
• Length: 289 a.a.
• NCBI ID: WP_060587255.1
• Uniprot ID: A0A0S2SLX4
• Organism: Aeromonas schubertii strain WL1483
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3471155..3482024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WL1483_RS22035	tapA	3471391..3471819 (+)	429	WP_082634874.1	type IVa pilus major pilin TapA	-
WL1483_RS16620 (WL1483_4313)	-	3471826..3472224 (+)	399	Protein_3239	type IV-A pilus assembly ATPase PilB	-
WL1483_RS16635 (WL1483_77)	pilB	3473557..3474870 (+)	1314	Protein_3241	type IV-A pilus assembly ATPase PilB	-
WL1483_RS16640 (WL1483_553)	pilC	3474891..3476129 (+)	1239	WP_050666599.1	type II secretion system F family protein	Machinery gene
WL1483_RS16645 (WL1483_3227)	pilD	3476155..3477024 (+)	870	WP_060587255.1	prepilin peptidase	Machinery gene
WL1483_RS16650 (WL1483_3670)	coaE	3477035..3477649 (+)	615	WP_060587256.1	dephospho-CoA kinase	-
WL1483_RS16655 (WL1483_3258)	zapD	3477688..3478410 (+)	723	WP_050666596.1	cell division protein ZapD	-
WL1483_RS16660 (WL1483_3909)	yacG	3478420..3478608 (+)	189	WP_050666595.1	DNA gyrase inhibitor YacG	-
WL1483_RS16665 (WL1483_2338)	mutT	3478640..3479050 (-)	411	WP_050666594.1	8-oxo-dGTP diphosphatase MutT	-
WL1483_RS16670 (WL1483_2949)	secA	3479113..3481824 (-)	2712	WP_060587257.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32156.48 Da
• Isoelectric Point: 7.1629

>NTDB_id=159544 WL1483_RS16645 WP_060587255.1 3476155..3477024(+) (pilD) [Aeromonas schubertii strain WL1483]
MTLLLQLSQAMPWLYYLLVALFALCVGSFLNVVIHRLPIMLERQWREEYRDYFTPDEATETAPFNLMVPRSRCPACGHGI
TAAENIPLLSWLWLRGRCSACKAPISVRYPLVELVTALLSLVVAWQLPAGWPTLAGLLLTWSLIPLTLIDLDRMLLPDQI
TLPLLWGGVLVNLVDGFVPLSDAVIGAMVGYLTLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPLILLLSALV
GAMVGITLILLRRHQQGNPIPFGPYLAMAGWIALLWGDGITRWYLSTVL

Nucleotide

• Length: 870 bp

>NTDB_id=159544 WL1483_RS16645 WP_060587255.1 3476155..3477024(+) (pilD) [Aeromonas schubertii strain WL1483]
ATGACCCTGTTGCTTCAACTCAGCCAGGCCATGCCCTGGCTCTACTATCTTCTGGTTGCGCTCTTTGCCCTCTGTGTCGG
CAGCTTCCTCAATGTGGTGATCCATCGCCTTCCCATCATGCTGGAGCGCCAGTGGCGCGAGGAGTATCGGGACTACTTCA
CCCCGGATGAAGCAACGGAGACCGCCCCCTTCAATCTGATGGTCCCGCGCTCCCGCTGCCCTGCCTGCGGTCACGGCATC
ACGGCCGCCGAGAATATTCCCCTGCTGAGCTGGCTCTGGTTACGCGGCCGTTGCAGTGCCTGTAAGGCACCTATTTCGGT
TCGCTACCCGCTGGTTGAGTTGGTCACGGCTCTGCTCTCACTGGTGGTGGCCTGGCAACTTCCCGCCGGTTGGCCCACCC
TGGCAGGGCTTCTGCTCACCTGGTCGCTTATCCCCCTGACCCTGATCGATCTGGATCGCATGCTACTGCCCGACCAGATC
ACCCTGCCCCTGCTCTGGGGAGGAGTACTGGTCAACCTTGTCGATGGCTTTGTCCCCCTCTCCGATGCGGTGATCGGCGC
CATGGTCGGATACCTGACCCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAAGAGGGGATGGGGTATGGGG
ACTTCAAACTGCTTGCCGCCCTTGGCGCCTGGCTCGGCTGGCAGGCACTACCCCTGATCCTGCTGCTCTCGGCACTGGTC
GGAGCCATGGTTGGTATCACGCTGATTCTGCTTCGCCGCCACCAGCAGGGTAATCCCATCCCCTTTGGCCCCTACCTCGC
CATGGCCGGGTGGATAGCCCTGCTCTGGGGTGACGGGATCACCCGCTGGTACCTCTCCACCGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

3D structure

• AlphaFold DB: A0A0S2SLX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	59.932	100	0.606
pilD	Vibrio campbellii strain DS40M4	53.952	100	0.543
pilD	Acinetobacter nosocomialis M2	52.03	93.772	0.488
pilD	Acinetobacter baumannii D1279779	51.661	93.772	0.484
pilD	Neisseria gonorrhoeae MS11	50.376	92.042	0.464