Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9P85_RS06595 Genome accession   NZ_CP016029
Coordinates   1471088..1472572 (-) Length   494 a.a.
NCBI ID   WP_011213621.1    Uniprot ID   I7HJU1
Organism   Legionella pneumophila strain Pontiac     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466088..1477572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P85_RS06580 (A9P85_06580) cysS 1467088..1468458 (+) 1371 WP_011215349.1 cysteine--tRNA ligase -
  A9P85_RS06585 (A9P85_06585) - 1468630..1470543 (+) 1914 WP_027220709.1 DUF3413 domain-containing protein -
  A9P85_RS06590 (A9P85_06590) - 1470673..1470987 (+) 315 WP_011213620.1 DUF6482 family protein -
  A9P85_RS06595 (A9P85_06595) pilB 1471088..1472572 (-) 1485 WP_011213621.1 GspE family T2SS ATPase variant LspE Machinery gene
  A9P85_RS06600 (A9P85_06600) lspD 1472576..1474951 (-) 2376 WP_011213622.1 GspD family T2SS secretin variant LspD -
  A9P85_RS06605 (A9P85_06605) - 1475124..1476152 (+) 1029 WP_027220708.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55121.45 Da        Isoelectric Point: 5.4872

>NTDB_id=158970 A9P85_RS06595 WP_011213621.1 1471088..1472572(-) (pilB) [Legionella pneumophila strain Pontiac]
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPATPSIREDGFKRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=158970 A9P85_RS06595 WP_011213621.1 1471088..1472572(-) (pilB) [Legionella pneumophila strain Pontiac]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCACTTACCTAACACACCACTGCAAGCTTTCGCTGAGATAAAGCGATTATTACAATGTGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCTGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCCGTCAGTGAAGACTTATTGGAAAA
CCAAGACGATGCCCCAATTATCCGCTTACTGAACGCTCTGTTTACCCAAGCGATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGCAATCGAATTGATGGTGTTCTACAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACATAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAACGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCCCATGGCATTATTTTAGTCACTGGCCCAACAGGTTCAGGCAAGACAACATCTCTTTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACACAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACCGCATTAGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGGCAGGAAGAA
AAGGAGTTAATGGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAGCCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCCGCCACGCCGAGCATACGTGAAGATGGTTTTAAAAGAGTATTATGTGGC
GATACGTCACTTGCAGAAATTTTAAGGGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7HJU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.067

96.356

0.415
  pilB Vibrio campbellii strain DS40M4

46.305

82.186

0.381
  pilB Acinetobacter baumannii D1279779

44.952

84.211

0.379
  pilB Vibrio parahaemolyticus RIMD 2210633

46.04

81.781

0.377
  pilB Acinetobacter baylyi ADP1

47.558

78.745

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

41.475

87.854

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36