Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   AO356_RS15925 Genome accession   NZ_CP012831
Coordinates   3601115..3602461 (-) Length   448 a.a.
NCBI ID   WP_060740568.1    Uniprot ID   A0A0N7H2B1
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3596115..3607461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS15910 (AO356_15890) - 3598038..3598439 (+) 402 WP_060740566.1 type IV pilin protein -
  AO356_RS15915 (AO356_15895) thiO 3598497..3599597 (+) 1101 WP_060740567.1 glycine oxidase ThiO -
  AO356_RS15920 (AO356_15900) - 3600748..3600951 (+) 204 Protein_3201 DUF6124 family protein -
  AO356_RS15925 (AO356_15905) pilR 3601115..3602461 (-) 1347 WP_060740568.1 sigma-54-dependent transcriptional regulator Regulator
  AO356_RS15930 (AO356_15910) pilS 3602458..3604047 (-) 1590 WP_060740569.1 ATP-binding protein Regulator
  AO356_RS15935 (AO356_15915) - 3604037..3604270 (-) 234 WP_060740570.1 PP0621 family protein -
  AO356_RS15940 (AO356_15920) - 3604396..3605412 (-) 1017 WP_046065043.1 outer membrane protein assembly factor BamD -
  AO356_RS15945 (AO356_15925) rluD 3605563..3606525 (+) 963 WP_060740571.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AO356_RS15950 (AO356_15930) pgeF 3606522..3607247 (+) 726 WP_060740572.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 49997.25 Da        Isoelectric Point: 5.5797

>NTDB_id=157820 AO356_RS15925 WP_060740568.1 3601115..3602461(-) (pilR) [Pseudomonas fluorescens strain FW300-N2C3]
MNNRSRQRILIVDDEPDIRELLDITLGRMKLDTRSAKNLAEAQALLTGDAFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAIDALKAGAFDFLTKPVDLGRLRELVTSALRLPPGATPTSTIERCLLGDSPPMRNVRKQIEKLARSQA
PVYISGESGCGKELVARLIHEQGPRASQSFVPVNCGAIPTELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVA
DLPLAMQVKLLRAIQEKAVRAVGGQQEEVVDVRILCATHKDLDGEVAAGRFRQDLYYRLNVIELRVPPLRERREDIEPLA
NHMLQRLASSTGSPAAKLHPQALEALQNYRFPGNVRELENMLERAYTLCEQQLIEADDLRLAEGNASSEAGNPDLMQVDN
LEDYLEDVERKLILQALEETRWNRTAAAQRLKLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=157820 AO356_RS15925 WP_060740568.1 3601115..3602461(-) (pilR) [Pseudomonas fluorescens strain FW300-N2C3]
TTGAATAATCGCTCACGGCAACGAATCCTGATCGTCGATGACGAACCGGACATCCGCGAACTCCTGGACATCACCCTGGG
CCGGATGAAACTCGACACCCGCAGCGCCAAGAACCTTGCCGAGGCCCAAGCCCTGCTGACGGGGGATGCCTTTGACCTGT
GCCTGACCGACATGCGCTTGCCCGATGGCACCGGCCTGGAACTGGTGCAGCACATCCAGCAACGTTATCCACAGCTGCCC
GTGGCGATGATCACCGCCTATGGCAGCCTGGAAACCGCCATCGACGCCCTCAAGGCCGGCGCTTTCGATTTCCTGACCAA
ACCGGTCGACCTGGGGCGGCTGCGGGAACTGGTCACCAGTGCCCTGCGCCTGCCGCCGGGCGCCACACCGACCAGCACCA
TCGAGCGCTGCCTGCTGGGCGACTCGCCGCCAATGCGCAACGTGCGCAAACAGATCGAAAAGCTCGCCCGCAGCCAGGCC
CCGGTGTACATCAGTGGAGAATCAGGCTGCGGCAAGGAACTGGTCGCCCGGCTGATCCACGAACAAGGCCCCCGCGCCAG
CCAGAGCTTTGTGCCGGTCAACTGTGGGGCAATTCCCACGGAACTGATGGAAAGCGAGTTCTTTGGCCACCGCAAAGGCA
GCTTCAGCGGTGCTATCGAAGACAAGCCAGGACTGTTCCAGGCTGCCCATGGCGGGACCTTGTTCCTCGATGAAGTGGCC
GACCTGCCCCTGGCGATGCAGGTCAAGCTGTTGCGAGCCATCCAGGAAAAGGCCGTACGCGCCGTGGGCGGCCAGCAGGA
AGAAGTGGTGGATGTGCGAATCCTCTGCGCCACCCACAAGGACCTGGACGGCGAAGTGGCCGCCGGGCGTTTTCGTCAGG
ACCTGTATTACCGGCTGAATGTCATCGAATTGCGCGTCCCGCCCTTGCGCGAACGCCGCGAAGACATCGAACCGCTGGCC
AATCACATGCTTCAGCGGCTGGCCAGCAGCACCGGCAGCCCGGCGGCCAAGCTACATCCACAAGCCCTGGAGGCCCTGCA
AAACTACCGTTTCCCGGGCAATGTGCGGGAACTGGAGAACATGCTCGAACGGGCCTACACCCTGTGCGAACAACAGCTGA
TCGAAGCCGACGACCTGCGCCTGGCCGAGGGCAATGCCAGCTCTGAGGCGGGCAACCCCGACTTGATGCAGGTCGACAAC
CTGGAAGACTACCTCGAAGACGTCGAACGTAAACTCATCCTCCAAGCCCTGGAGGAAACCCGCTGGAACCGCACGGCGGC
GGCGCAGCGGTTGAAGTTGTCGTTTCGGTCGATGCGGTATCGGTTGAAGAAGTTGGGGCTGGATTAA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(139-304)

Bacterial regulatory protein, Fis family (HTH_8)

(405-445)

Response regulator receiver domain (Response_reg)

(9-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N7H2B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

78.604

99.107

0.779
  pilR Acinetobacter baumannii strain A118

49.02

100

0.502

Multiple sequence alignment