Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AO356_RS15930 Genome accession   NZ_CP012831
Coordinates   3602458..3604047 (-) Length   529 a.a.
NCBI ID   WP_060740569.1    Uniprot ID   A0A0N9W952
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3597458..3609047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS15910 (AO356_15890) - 3598038..3598439 (+) 402 WP_060740566.1 type IV pilin protein -
  AO356_RS15915 (AO356_15895) thiO 3598497..3599597 (+) 1101 WP_060740567.1 glycine oxidase ThiO -
  AO356_RS15920 (AO356_15900) - 3600748..3600951 (+) 204 Protein_3201 DUF6124 family protein -
  AO356_RS15925 (AO356_15905) pilR 3601115..3602461 (-) 1347 WP_060740568.1 sigma-54-dependent transcriptional regulator Regulator
  AO356_RS15930 (AO356_15910) pilS 3602458..3604047 (-) 1590 WP_060740569.1 ATP-binding protein Regulator
  AO356_RS15935 (AO356_15915) - 3604037..3604270 (-) 234 WP_060740570.1 PP0621 family protein -
  AO356_RS15940 (AO356_15920) - 3604396..3605412 (-) 1017 WP_046065043.1 outer membrane protein assembly factor BamD -
  AO356_RS15945 (AO356_15925) rluD 3605563..3606525 (+) 963 WP_060740571.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AO356_RS15950 (AO356_15930) pgeF 3606522..3607247 (+) 726 WP_060740572.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58824.58 Da        Isoelectric Point: 7.3785

>NTDB_id=157821 AO356_RS15930 WP_060740569.1 3602458..3604047(-) (pilS) [Pseudomonas fluorescens strain FW300-N2C3]
MSAEASSPRVKQTQRLLRLYHLYRLSIGITLVLLISSNMDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGVPSAIGNLLIVSVAIGNTLLRGRIGLLIAAVATLGIVGSSLFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQPLD
DYSTALVERLQLWRNNPSLRPSSLTVTGTGLTLQPSFIALGHHDQQQILVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNNADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRTWLDQFVRQARESMAEH
QQLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMVHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=157821 AO356_RS15930 WP_060740569.1 3602458..3604047(-) (pilS) [Pseudomonas fluorescens strain FW300-N2C3]
GTGAGCGCTGAGGCCTCAAGCCCACGCGTCAAACAGACGCAGCGCCTGCTGCGCCTCTATCACCTGTACCGCCTGAGTAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGGAGTTCGCCAACGACGACCTGCTGCGCA
GCGGCAGTTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTTGAGAACACGCGTCGCCCTGCCCGGCTGTTC
GGCCTGGCCCTCACCGACGTGCTGCTGCTTTCGTGGCTGTTTTTCGCCGCCGGCGGCGTGCCCAGTGCCATCGGCAACCT
GCTCATTGTCTCGGTGGCCATCGGCAACACGCTGCTGCGAGGTCGGATCGGCCTGTTGATCGCGGCCGTTGCCACCCTCG
GCATCGTCGGTTCGAGCCTCTTTCTTGGCTTGAGCGACTCGAACCGCCCCAGCAGTTATTTGCAGGCCGGCACCCTCGGG
GCACTGTGCTTTGCCGCCGCGCTGCTGGTGCAAGGCCTGACCCGACGCTTGGAAGCCAGCGAAACCCTGGCCGAGCAACG
GGCCAGCGAAGTGATCGGCCTGGAAGCGCTCAACGCCCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTTGACC
GTGAGCGCCGGGTGCAACTGGCCAACGAGAGTGCGCTGAACCTGCTGGGCATGCACGATCTGGTCGGCCAGCCGCTCGAC
GATTACTCCACTGCCCTGGTCGAACGCCTGCAATTGTGGCGAAACAACCCCAGCCTGCGCCCGTCCAGCCTGACCGTTAC
GGGCACGGGCCTGACCCTGCAACCGAGCTTCATCGCCCTGGGGCATCACGATCAGCAGCAGATCCTGGTGTTCCTTGAAG
ACCTGGCCCAGGTTGCCCAGCAGGCCCAGCAGTTGAAACTCGCGTCCCTCGGGCGCCTCACCGCCGGTATCGCCCATGAA
ATCCGCAACCCCTTGGGCGCCATCAGTCACGCGGCGCAATTGCTACGCGAATCCGAGGAACTGAACAACGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAATCGCGTCATTGAAAACGTTCTGCAGCTGTCTCGCCGCCAAC
AAACCACACCCCAGCGCCTCGACCTGCGCACCTGGCTCGACCAATTCGTCCGGCAGGCCCGCGAAAGCATGGCCGAACAC
CAGCAATTGCACCTGAGCATCGACCCGGGGGACTACACCACGCTGATGGACCCCGACCAGCTCACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCAATGGTCCATGAACAGGCCGAGGCCTGGTTGAAACTGTTCATCGACCCCC
ACAGCCAGCTGTCGACACTGGACATCATCGACAACGGCCCCGGTGTAACGCCCGAGCAGCAGGCGCACTTGTTCGAACCC
TTCTTTACCACCAGTAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAAGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGTTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N9W952

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.227

99.811

0.601

Multiple sequence alignment