Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   AO353_RS14200 Genome accession   NZ_CP012830
Coordinates   3153128..3154474 (-) Length   448 a.a.
NCBI ID   WP_054595526.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3148128..3159474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS14185 (AO353_14185) - 3148339..3151500 (+) 3162 WP_054595523.1 pilus assembly protein -
  AO353_RS14190 (AO353_14190) - 3151516..3151917 (+) 402 WP_054595524.1 type IV pilin protein -
  AO353_RS14195 (AO353_14195) thiO 3151975..3153075 (+) 1101 WP_054595525.1 glycine oxidase ThiO -
  AO353_RS14200 (AO353_14200) pilR 3153128..3154474 (-) 1347 WP_054595526.1 sigma-54-dependent transcriptional regulator Regulator
  AO353_RS14205 (AO353_14205) pilS 3154471..3156060 (-) 1590 WP_054595527.1 sensor histidine kinase Regulator
  AO353_RS14210 (AO353_14210) - 3156050..3156283 (-) 234 WP_054595528.1 PP0621 family protein -
  AO353_RS14215 (AO353_14215) - 3156408..3157424 (-) 1017 WP_054595529.1 outer membrane protein assembly factor BamD -
  AO353_RS14220 (AO353_14220) rluD 3157577..3158539 (+) 963 WP_054595530.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AO353_RS14225 (AO353_14225) pgeF 3158536..3159261 (+) 726 WP_054595531.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 49879.42 Da        Isoelectric Point: 7.4253

>NTDB_id=157785 AO353_RS14200 WP_054595526.1 3153128..3154474(-) (pilR) [Pseudomonas fluorescens strain FW300-N2E3]
MKTSPRQKVLIVDDEPDIRELLEITLGRMKLDTLSARNVGEARSLLAQERFDLCLTDMRLPDGTGLELVQHIQLTHPYVP
VAMITAYGSLDTAINALKAGAFDFLTKPVNLTRLRELVVSALRLPVAGDAASAIDRRLLGDSVPMRNLRTQIDKLARSQA
PVYISGESGSGKELVARLIHEQGPRSGQPFVPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAANGGTLFLDEVA
DLPLAMQVKLLRAIQEKAVRSIGGQQEAIINVRILCATHRDLDAEVAAGRFRQDLYYRLNVIELRVPPLRERRDDIELLA
SHVLKRLAAGSGLPAVHLQPQALDALKHYRFPGNVRELENMLERAYTLCENKQIEASDLRLTDGNCSADTGLPDLTKIDN
LEQYLENVERKLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=157785 AO353_RS14200 WP_054595526.1 3153128..3154474(-) (pilR) [Pseudomonas fluorescens strain FW300-N2E3]
TTGAAGACAAGCCCACGGCAAAAAGTCCTGATCGTCGATGACGAACCGGATATTCGCGAACTTCTGGAAATCACCCTCGG
CCGAATGAAACTCGATACGCTGAGCGCGCGTAATGTCGGCGAAGCGCGGTCGTTGCTGGCCCAGGAGCGGTTCGACCTGT
GCCTGACCGATATGCGGTTGCCCGACGGTACCGGCCTGGAGCTGGTGCAGCACATTCAGCTGACCCATCCCTACGTGCCT
GTGGCGATGATCACCGCTTATGGCAGCCTTGATACCGCCATCAACGCGCTGAAAGCCGGTGCCTTCGACTTCCTGACCAA
ACCGGTGAACCTCACCCGACTGCGCGAGCTGGTGGTCAGCGCCCTGCGCTTGCCGGTCGCGGGCGACGCTGCCAGTGCCA
TCGATCGCCGGCTGCTCGGCGACTCAGTGCCCATGCGCAACCTGCGCACACAAATCGACAAACTGGCGCGCAGCCAGGCA
CCGGTGTACATCAGCGGCGAATCCGGCAGTGGAAAGGAGCTGGTCGCCCGACTGATTCATGAACAAGGTCCACGCAGTGG
CCAGCCATTTGTGCCGGTCAACTGCGGGGCAATTCCGTCCGAATTGATGGAAAGCGAGTTTTTCGGTCATCGCAAAGGCA
GTTTCAGCGGCGCCATCGAAGACAAACCCGGGCTGTTCCAGGCGGCTAACGGTGGGACGTTGTTTCTCGATGAAGTGGCC
GACTTGCCGCTGGCGATGCAGGTCAAGTTGCTGCGCGCGATTCAGGAGAAGGCGGTACGCAGCATCGGCGGACAGCAGGA
AGCCATCATTAATGTGCGAATACTCTGTGCGACCCACAGGGATCTGGACGCCGAAGTGGCCGCTGGGCGCTTTCGGCAGG
ATTTGTACTATCGGCTGAATGTCATCGAGTTGCGCGTCCCGCCGCTACGCGAGCGCCGTGACGACATCGAGCTGCTGGCC
AGTCATGTACTCAAGCGTCTCGCGGCAGGCAGCGGCTTGCCGGCCGTCCATCTCCAGCCGCAAGCCCTCGATGCACTCAA
GCACTACCGTTTTCCGGGTAACGTCCGCGAGCTGGAAAACATGCTCGAACGCGCGTACACCCTGTGCGAAAACAAGCAGA
TCGAAGCCAGCGACCTGCGCCTGACTGATGGCAATTGCAGCGCCGATACGGGATTGCCGGACCTGACGAAAATCGACAAC
CTTGAGCAGTATCTGGAGAACGTCGAACGCAAACTGATTCTCCAGGCGCTCGAAGAAACCCGCTGGAACCGCACAGCGGC
GGCCCAACGGCTGAACCTGTCGTTTCGGTCGATGCGTTACCGGCTGAAAAAGCTGGGGCTGGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(9-118)

Bacterial regulatory protein, Fis family (HTH_8)

(405-445)

Sigma-54 interaction domain (Sigma54_activat)

(139-304)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

79.007

98.884

0.781
  pilR Acinetobacter baumannii strain A118

50.109

100

0.513

Multiple sequence alignment