Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AO353_RS14205 Genome accession   NZ_CP012830
Coordinates   3154471..3156060 (-) Length   529 a.a.
NCBI ID   WP_054595527.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3149471..3161060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS14190 (AO353_14190) - 3151516..3151917 (+) 402 WP_054595524.1 type IV pilin protein -
  AO353_RS14195 (AO353_14195) thiO 3151975..3153075 (+) 1101 WP_054595525.1 glycine oxidase ThiO -
  AO353_RS14200 (AO353_14200) pilR 3153128..3154474 (-) 1347 WP_054595526.1 sigma-54-dependent transcriptional regulator Regulator
  AO353_RS14205 (AO353_14205) pilS 3154471..3156060 (-) 1590 WP_054595527.1 sensor histidine kinase Regulator
  AO353_RS14210 (AO353_14210) - 3156050..3156283 (-) 234 WP_054595528.1 PP0621 family protein -
  AO353_RS14215 (AO353_14215) - 3156408..3157424 (-) 1017 WP_054595529.1 outer membrane protein assembly factor BamD -
  AO353_RS14220 (AO353_14220) rluD 3157577..3158539 (+) 963 WP_054595530.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AO353_RS14225 (AO353_14225) pgeF 3158536..3159261 (+) 726 WP_054595531.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58342.11 Da        Isoelectric Point: 6.8230

>NTDB_id=157786 AO353_RS14205 WP_054595527.1 3154471..3156060(-) (pilS) [Pseudomonas fluorescens strain FW300-N2E3]
MIAEISSPGDKQAQRLLRLYHLYRLSIGLLLVLLISSNMDNQLLEFTNDDLLRNGSWLYLILNILLVVFAGNPRQPAQIF
GLTLVDILLLSVLFYAAGGVPSAIGNLLIVSVAIGNTLLRRRIGLLLAAVAAIGIISLTFSLSGSHPTGANHYLQAGTLG
ALCFAAALLVQGLTRQLEVSESLAQQRASEVVSLEALNALILQRMRTGILVLDSERRVQLANHSALSLFGQTHLDGQLID
DYSTALVERLQLWFNNPTLRPQSLKVARTGLELQPSFIALGLNEQRQTLVFLEDLAQVAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLNESEELIGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQVVPQRLDLKQWLEQFVCTVREGATDR
QQIHLNAGPGPFTTLIDPDQLTQVLDNLLRNAWRHSALAHEQAEVWLTLFIDPDSQLPTLDVLDNGPGVSAEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=157786 AO353_RS14205 WP_054595527.1 3154471..3156060(-) (pilS) [Pseudomonas fluorescens strain FW300-N2E3]
GTGATCGCTGAGATCTCGAGCCCTGGCGACAAACAGGCCCAGCGACTGCTGCGCCTCTATCATCTCTACCGTTTAAGCAT
CGGCCTCCTTCTGGTGCTGCTGATCTCCAGCAACATGGACAACCAGTTGCTGGAGTTCACCAACGACGACCTGCTGCGCA
ATGGCAGTTGGTTGTACCTGATTCTGAATATCCTGCTGGTGGTATTTGCGGGAAACCCCAGACAACCCGCACAGATCTTC
GGCCTCACGCTGGTTGATATCCTGCTGTTATCAGTGCTGTTCTATGCCGCAGGTGGCGTCCCCAGCGCCATCGGCAATCT
GCTGATCGTGTCCGTGGCGATCGGCAATACACTGCTGCGCCGGCGCATCGGCCTGCTGCTCGCGGCAGTCGCCGCCATCG
GTATCATTTCCCTGACTTTCTCCCTGAGCGGCAGCCACCCGACTGGCGCCAATCACTACTTGCAAGCCGGCACCCTGGGC
GCCCTGTGCTTTGCCGCAGCCTTGCTGGTGCAAGGCCTGACCCGACAACTGGAGGTCAGCGAGAGTCTGGCCCAGCAGCG
CGCCAGCGAAGTGGTCAGCCTGGAAGCGCTCAATGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTACTCGACA
GTGAGCGCCGGGTTCAGTTGGCCAATCACAGCGCCTTGAGTCTGTTTGGCCAGACACACCTCGACGGCCAACTCATCGAC
GATTATTCGACAGCCTTGGTCGAGCGCCTGCAACTCTGGTTCAACAACCCGACCTTGCGCCCACAAAGCCTGAAAGTCGC
CCGCACTGGCCTGGAACTGCAACCGAGCTTCATCGCGCTGGGCCTGAACGAACAACGCCAGACCCTGGTATTTCTCGAAG
ACCTTGCCCAGGTCGCGCAACAAGCCCAGCAACTCAAGCTTGCAGCACTGGGGCGGCTGACCGCAGGCATTGCCCATGAA
ATCCGCAACCCATTGGGTGCCATCAGCCATGCGGCGCAGTTGCTGAACGAGTCTGAGGAACTGATCGGCGCCGACCGGCG
TCTGACGCAGATTATTCAGGACCATTCTCAACGCATGAACCGAGTGATAGAAAACGTCCTGCAGCTTTCACGGCGCCAGC
AAGTCGTACCGCAACGGCTCGACTTGAAGCAGTGGCTGGAGCAGTTCGTGTGCACGGTGCGCGAAGGAGCAACGGATCGC
CAACAGATTCATCTGAACGCCGGACCAGGCCCCTTCACCACCTTGATAGACCCAGACCAACTGACTCAGGTGCTCGATAA
CCTGTTGCGCAATGCCTGGCGCCACAGCGCCCTTGCGCATGAACAGGCCGAAGTCTGGCTCACGCTGTTTATCGACCCCG
ACAGTCAGCTTCCAACCCTGGATGTGCTGGACAATGGGCCCGGCGTTTCAGCGGAGCAGCAAGCACATTTGTTCGAACCG
TTCTTCACCACCAGTAGCCAGGGTACTGGCCTGGGTCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.245

100

0.594

Multiple sequence alignment