Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AOT13_RS16685 Genome accession   NZ_CP012712
Coordinates   3385538..3386602 (-) Length   354 a.a.
NCBI ID   WP_003249181.1    Uniprot ID   -
Organism   Parageobacillus thermoglucosidasius strain DSM 2542     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3380538..3391602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT13_RS16640 (AOT13_16620) - 3381349..3382140 (+) 792 WP_003249197.1 YqhG family protein -
  AOT13_RS16645 (AOT13_16625) - 3382198..3382377 (-) 180 WP_003249195.1 YqzE family protein -
  AOT13_RS16655 (AOT13_16635) comGG 3382643..3383032 (-) 390 WP_003249192.1 competence type IV pilus minor pilin ComGG -
  AOT13_RS16660 (AOT13_16640) comGF 3383049..3383474 (-) 426 WP_003249190.1 competence type IV pilus minor pilin ComGF -
  AOT13_RS16665 (AOT13_16645) comGE 3383471..3383794 (-) 324 WP_003249188.1 competence type IV pilus minor pilin ComGE -
  AOT13_RS16670 (AOT13_16650) comGD 3383778..3384221 (-) 444 WP_003249186.1 competence type IV pilus minor pilin ComGD -
  AOT13_RS16675 (AOT13_16655) comGC 3384205..3384501 (-) 297 WP_003249184.1 competence type IV pilus major pilin ComGC Machinery gene
  AOT13_RS16680 (AOT13_16660) comGB 3384514..3385545 (-) 1032 WP_003249183.1 competence type IV pilus assembly protein ComGB -
  AOT13_RS16685 (AOT13_16665) comGA 3385538..3386602 (-) 1065 WP_003249181.1 competence type IV pilus ATPase ComGA Machinery gene
  AOT13_RS16690 (AOT13_16670) - 3386803..3387501 (+) 699 WP_003249180.1 helix-turn-helix domain-containing protein -
  AOT13_RS16695 (AOT13_16675) - 3387701..3387943 (+) 243 WP_003249178.1 DUF2626 family protein -
  AOT13_RS16700 (AOT13_16680) - 3387994..3389127 (-) 1134 WP_003249175.1 SAM-dependent methyltransferase -
  AOT13_RS16705 (AOT13_16685) - 3389458..3390090 (-) 633 WP_003249173.1 MBL fold metallo-hydrolase -
  AOT13_RS19185 - 3390427..3390603 (+) 177 WP_013400388.1 DUF2759 domain-containing protein -
  AOT13_RS16710 (AOT13_16690) - 3390636..3390947 (-) 312 WP_003249171.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39888.38 Da        Isoelectric Point: 9.1523

>NTDB_id=156708 AOT13_RS16685 WP_003249181.1 3385538..3386602(-) (comGA) [Parageobacillus thermoglucosidasius strain DSM 2542]
MNEIEYLADRLIKEASVLRVSDIHIVPRKDDAAVRFRLDGLLMEKEVLTKEICDRLITHFKFLAGMDIGERRRPQSGAME
TKQQGEIIHLRLSTLPTSYDESLVIRLLPQNLFLPLSQLSLFANAAKTLLSLFQQPQGLIIFTGPTGSGKTTTLYTLLRV
CQSKWNRNVITLEDPVEKRIDNILQVQINEKAGITYANGLKAALRHDPNIIMIGEIRDAETAKIAVRSSMTGHLIASTMH
TKNAVGAIYRLREFGIPLHDIEQTLLAVTAQRLVDLVCPFCGEHCSPFCRKHRHIRRAAVHELLYGDALSDAIQSVQNGR
KTYRYYTLQNVIRKGVALGFLPPRLLCAKEGENE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=156708 AOT13_RS16685 WP_003249181.1 3385538..3386602(-) (comGA) [Parageobacillus thermoglucosidasius strain DSM 2542]
ATGAATGAAATCGAATATCTTGCCGACCGTCTCATCAAAGAAGCAAGTGTGCTTCGTGTGTCTGACATTCACATCGTTCC
GCGCAAAGATGATGCAGCAGTGCGTTTCCGGCTTGATGGATTGCTTATGGAAAAAGAAGTGCTCACAAAAGAAATATGCG
ATCGCCTGATAACCCATTTTAAGTTTTTAGCCGGAATGGACATTGGCGAACGCCGCCGGCCGCAAAGCGGGGCGATGGAA
ACAAAGCAGCAAGGGGAAATTATTCACTTGCGCCTTTCCACACTGCCGACATCTTACGATGAAAGCCTCGTCATTCGGCT
TCTTCCCCAAAATTTGTTTCTTCCTCTTTCACAGCTTTCTTTATTTGCCAATGCCGCAAAAACATTGCTTTCCCTTTTTC
AGCAGCCTCAAGGGTTAATTATTTTCACAGGACCAACCGGGTCAGGAAAGACGACGACATTGTACACGTTGCTGCGCGTC
TGCCAGTCCAAATGGAACCGCAATGTCATCACATTGGAGGATCCTGTTGAAAAGCGAATCGACAATATATTGCAAGTGCA
AATCAATGAGAAAGCAGGCATCACATATGCAAACGGCTTAAAAGCAGCTTTGCGGCACGATCCAAACATTATTATGATCG
GCGAAATCCGGGATGCCGAAACGGCAAAAATTGCGGTACGTTCGTCGATGACAGGACATTTGATTGCGTCGACAATGCAT
ACGAAAAACGCGGTGGGAGCGATTTACCGTTTGCGTGAATTTGGGATTCCGCTTCATGATATTGAGCAAACGCTGCTTGC
CGTGACAGCGCAGCGGCTTGTCGACTTAGTATGCCCGTTTTGCGGCGAACATTGCTCGCCATTTTGCCGCAAACATCGCC
ACATTCGCCGCGCCGCCGTTCATGAGCTGTTGTATGGAGATGCGCTGTCTGACGCAATTCAATCCGTGCAAAACGGAAGA
AAAACGTATCGTTACTATACGCTGCAAAACGTGATTCGAAAAGGGGTTGCTCTCGGATTTTTGCCGCCGCGCCTTCTTTG
CGCCAAGGAGGGAGAAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.433

96.61

0.545
  pilB Haemophilus influenzae 86-028NP

41.562

90.395

0.376
  pilB Glaesserella parasuis strain SC1401

42.434

85.876

0.364

Multiple sequence alignment