Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NB74_RS07655 Genome accession   NZ_CP012593
Coordinates   1582327..1582824 (-) Length   165 a.a.
NCBI ID   WP_029650985.1    Uniprot ID   -
Organism   Staphylococcus aureus strain HOU1444-VR     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1577327..1587824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB74_RS07630 (AM595_07740) gcvPB 1577489..1578961 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  NB74_RS07635 (AM595_07745) gcvPA 1578954..1580300 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NB74_RS07640 (AM595_07750) gcvT 1580320..1581411 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NB74_RS07645 (AM595_07755) - 1581570..1582094 (-) 525 WP_001015120.1 shikimate kinase -
  NB74_RS07650 (AM595_07760) - 1582084..1582230 (-) 147 WP_001792109.1 hypothetical protein -
  NB74_RS07655 (AM595_07765) comGF 1582327..1582824 (-) 498 WP_029650985.1 competence type IV pilus minor pilin ComGF Machinery gene
  NB74_RS07660 (AM595_07770) comGE 1582742..1583041 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  NB74_RS07665 (AM595_07775) comGD 1583028..1583474 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NB74_RS07670 (AM595_07780) comGC 1583452..1583763 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NB74_RS07675 (AM595_07785) comGB 1583777..1584847 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NB74_RS07680 (AM595_07790) comGA 1584819..1585793 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NB74_RS07685 (AM595_07795) - 1585845..1586468 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NB74_RS07690 (AM595_07800) - 1586465..1586794 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NB74_RS07695 (AM595_07805) - 1586794..1587780 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.05 Da        Isoelectric Point: 10.3787

>NTDB_id=155686 NB74_RS07655 WP_029650985.1 1582327..1582824(-) (comGF) [Staphylococcus aureus strain HOU1444-VR]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=155686 NB74_RS07655 WP_029650985.1 1582327..1582824(-) (comGF) [Staphylococcus aureus strain HOU1444-VR]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

99.394

100

0.994
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment