Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   A8714_RS18410 Genome accession   NZ_CP015853
Coordinates   3598171..3600252 (-) Length   693 a.a.
NCBI ID   WP_000147776.1    Uniprot ID   Q8XD86
Organism   Escherichia coli strain ATCC 43889     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3593171..3605252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8714_RS18395 (A8714_18515) xanP 3594441..3595832 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  A8714_RS18400 (A8714_18520) gltS 3596112..3597317 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  A8714_RS18405 (A8714_18525) - 3597320..3598186 (+) 867 WP_000747329.1 hypothetical protein -
  A8714_RS18410 (A8714_18530) recG 3598171..3600252 (-) 2082 WP_000147776.1 ATP-dependent DNA helicase RecG Machinery gene
  A8714_RS18415 (A8714_18535) trmH 3600258..3600947 (-) 690 WP_001070177.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  A8714_RS18420 (A8714_18540) spoT 3600954..3603062 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  A8714_RS18425 (A8714_18545) rpoZ 3603081..3603356 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  A8714_RS18430 (A8714_18550) gmk 3603411..3604034 (-) 624 WP_001301691.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76496.38 Da        Isoelectric Point: 7.0947

>NTDB_id=155368 A8714_RS18410 WP_000147776.1 3598171..3600252(-) (recG) [Escherichia coli strain ATCC 43889]
MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=155368 A8714_RS18410 WP_000147776.1 3598171..3600252(-) (recG) [Escherichia coli strain ATCC 43889]
ATGACAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGCACGCCAGAATTACAGGAAACGCTCACGCCGGTTTATCCAACAACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCACCGACGCTACAGCTTAGCGATCTGG
AAACCGGGCAGCATCCAGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCACAACCTCAGCATGTTAGCCTTACGT
GCCGGAGCACAGCGTTTTCATGCCCAGCCGCTGAGCGCCAATGATGCGCTGAAAAATAAACTCCTCGCAGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGCAACTGGTTTGAACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGCAAAGCGCGACTGTCTCAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGTCTGGCGCTGGTGATTATCGACGAACAACAT
CGCTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCAATGACGGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATA
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAATTGAATGTTGGCCTGGTACACGGGCGGATGAAACCAGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACATCTGCTAGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGCCTGATGATTATCGAAAACCCTGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGGCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTGAGTTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAAGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCCAACGCAT
GA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

DEAD/DEAH box helicase (DEAD)

(273-433)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8XD86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.53

96.97

0.509
  recG/mmsA Streptococcus pneumoniae R6

39.074

99.711

0.39
  recG/mmsA Streptococcus pneumoniae R36A

39.074

99.711

0.39
  recG Bacillus subtilis subsp. subtilis str. 168

39.362

94.949

0.374

Multiple sequence alignment