Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A8V30_RS22010 Genome accession   NZ_CP015846
Coordinates   4032693..4033352 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O157:H7 strain FRIK2069     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4027693..4038352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8V30_RS21995 (A8V30_20955) ygiS 4029898..4031505 (-) 1608 WP_001301989.1 ABC transporter substrate-binding protein -
  A8V30_RS22000 (A8V30_20960) ygiV 4031614..4032096 (-) 483 WP_000183479.1 AraC family transcriptional regulator -
  A8V30_RS22005 (A8V30_20965) ygiW 4032149..4032541 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A8V30_RS22010 (A8V30_20970) ciaR 4032693..4033352 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  A8V30_RS22015 (A8V30_20975) qseC 4033349..4034698 (+) 1350 WP_000673362.1 quorum sensing histidine kinase QseC -
  A8V30_RS22020 (A8V30_20980) mdaB 4034808..4035389 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  A8V30_RS22025 (A8V30_20985) ygiN 4035420..4035734 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  A8V30_RS22030 (A8V30_20990) - 4035779..4036666 (-) 888 WP_000614950.1 MurR/RpiR family transcriptional regulator -
  A8V30_RS22035 (A8V30_20995) - 4036663..4037610 (-) 948 WP_001301797.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=155211 A8V30_RS22010 WP_001221502.1 4032693..4033352(+) (ciaR) [Escherichia coli O157:H7 strain FRIK2069]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=155211 A8V30_RS22010 WP_001221502.1 4032693..4033352(+) (ciaR) [Escherichia coli O157:H7 strain FRIK2069]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment