Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   WM90_RS24140 Genome accession   NZ_CP015159
Coordinates   4863638..4864297 (+) Length   219 a.a.
NCBI ID   WP_001546136.1    Uniprot ID   -
Organism   Escherichia coli strain Eco889     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4858638..4869297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM90_RS24125 (WM90_24135) ygiS 4860843..4862450 (-) 1608 WP_001468194.1 ABC transporter substrate-binding protein -
  WM90_RS24130 (WM90_24140) ygiV 4862559..4863041 (-) 483 WP_000183493.1 AraC family transcriptional regulator -
  WM90_RS24135 (WM90_24145) ygiW 4863094..4863486 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  WM90_RS24140 (WM90_24150) ciaR 4863638..4864297 (+) 660 WP_001546136.1 quorum sensing response regulator transcription factor QseB Regulator
  WM90_RS24145 (WM90_24155) qseC 4864294..4865643 (+) 1350 WP_001546137.1 quorum sensing histidine kinase QseC -
  WM90_RS24150 (WM90_24160) ygiZ 4865689..4866021 (-) 333 WP_000914696.1 DUF2645 family protein -
  WM90_RS31110 (WM90_24165) - 4866028..4866738 (-) 711 WP_000834022.1 hypothetical protein -
  WM90_RS31115 (WM90_24170) - 4866741..4867220 (-) 480 WP_000065331.1 Hcp family type VI secretion system effector -
  WM90_RS24165 (WM90_24175) mdaB 4867470..4868051 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  WM90_RS24170 (WM90_24180) ygiN 4868082..4868396 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24615.56 Da        Isoelectric Point: 7.4216

>NTDB_id=152192 WM90_RS24140 WP_001546136.1 4863638..4864297(+) (ciaR) [Escherichia coli strain Eco889]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWRGKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=152192 WM90_RS24140 WP_001546136.1 4863638..4864297(+) (ciaR) [Escherichia coli strain Eco889]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGGAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361