Detailed information NTG0150178

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: SAHC1340_RS09070
• Genome accession: NZ_CP012011
• Coordinates: 1782015..1782512 (-)
• Length: 165 a.a.
• NCBI ID: WP_001803317.1
• Uniprot ID: A0A2I7Y542
• Organism: Staphylococcus aureus strain HC1340
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1777015..1787512

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAHC1340_RS09045 (SAHC1340_02475)	gcvPB	1777177..1778649 (-)	1473	WP_000202189.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
SAHC1340_RS09050 (SAHC1340_02476)	gcvPA	1778642..1779988 (-)	1347	WP_042857059.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
SAHC1340_RS09055 (SAHC1340_02477)	gcvT	1780008..1781099 (-)	1092	WP_000093351.1	glycine cleavage system aminomethyltransferase GcvT	-
SAHC1340_RS09060 (SAHC1340_02478)	-	1781258..1781782 (-)	525	WP_001015117.1	shikimate kinase	-
SAHC1340_RS09065	-	1781772..1781918 (-)	147	WP_001789879.1	hypothetical protein	-
SAHC1340_RS09070 (SAHC1340_02479)	comGF	1782015..1782512 (-)	498	WP_001803317.1	competence type IV pilus minor pilin ComGF	Machinery gene
SAHC1340_RS09075 (SAHC1340_02480)	comGE	1782430..1782729 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
SAHC1340_RS09080 (SAHC1340_02481)	comGD	1782716..1783162 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
SAHC1340_RS09085 (SAHC1340_02482)	comGC	1783140..1783451 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
SAHC1340_RS09090 (SAHC1340_02483)	comGB	1783465..1784535 (-)	1071	WP_000775708.1	competence type IV pilus assembly protein ComGB	Machinery gene
SAHC1340_RS09095 (SAHC1340_02484)	comGA	1784507..1785481 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
SAHC1340_RS09100 (SAHC1340_02485)	-	1785533..1786156 (-)	624	WP_042857058.1	MBL fold metallo-hydrolase	-
SAHC1340_RS09105 (SAHC1340_02486)	-	1786153..1786482 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
SAHC1340_RS09110 (SAHC1340_02487)	-	1786482..1787468 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19212.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=150178 SAHC1340_RS09070 WP_001803317.1 1782015..1782512(-) (comGF) [Staphylococcus aureus strain HC1340]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=150178 SAHC1340_RS09070 WP_001803317.1 1782015..1782512(-) (comGF) [Staphylococcus aureus strain HC1340]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

3D structure

• AlphaFold DB: A0A2I7Y542

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	99.394	100	0.994
comGF	Staphylococcus aureus N315	95.758	100	0.958