Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PA50071_RS23660 Genome accession   NZ_CP012001
Coordinates   5133499..5134836 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5128499..5139836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA50071_RS23635 (PA50071_23645) pgeF 5128642..5129370 (-) 729 WP_012614543.1 peptidoglycan editing factor PgeF -
  PA50071_RS23640 (PA50071_23650) rluD 5129367..5130329 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PA50071_RS23645 (PA50071_23655) - 5130475..5131500 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PA50071_RS23650 (PA50071_23660) - 5131657..5131902 (+) 246 WP_003102591.1 PP0621 family protein -
  PA50071_RS23655 (PA50071_23665) pilS 5131892..5133484 (+) 1593 WP_003110428.1 two-component system sensor histidine kinase PilS Regulator
  PA50071_RS23660 (PA50071_23670) pilR 5133499..5134836 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  PA50071_RS23665 (PA50071_23675) thiO 5134882..5135976 (-) 1095 WP_031801325.1 glycine oxidase ThiO -
  PA50071_RS23670 (PA50071_23680) - 5136111..5136620 (+) 510 WP_003102595.1 GspH/FimT family pseudopilin -
  PA50071_RS23675 (PA50071_23685) - 5136726..5137232 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  PA50071_RS23680 (PA50071_23690) pilV 5137223..5137780 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  PA50071_RS23685 (PA50071_23695) pilW 5137777..5138601 (+) 825 WP_048521676.1 type 4a pilus minor pilin PilW -
  PA50071_RS23690 (PA50071_23700) pilX 5138598..5139185 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=150016 PA50071_RS23660 WP_003094694.1 5133499..5134836(+) (pilR) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=150016 PA50071_RS23660 WP_003094694.1 5133499..5134836(+) (pilR) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGTGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCACCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAAAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment