Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AA961_RS07535 Genome accession   NZ_CP011528
Coordinates   1530661..1531635 (-) Length   324 a.a.
NCBI ID   WP_001633882.1    Uniprot ID   -
Organism   Staphylococcus aureus strain RKI4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1525661..1536635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA961_RS07495 (AA961_07495) gcvT 1526162..1527253 (-) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  AA961_RS07500 (AA961_07500) - 1527412..1527936 (-) 525 WP_001015120.1 shikimate kinase -
  AA961_RS14715 (AA961_07505) - 1527926..1528072 (-) 147 WP_001792109.1 hypothetical protein -
  AA961_RS07510 (AA961_07510) comGF 1528169..1528666 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  AA961_RS07515 (AA961_07515) comGE 1528584..1528883 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AA961_RS07520 (AA961_07520) comGD 1528870..1529316 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  AA961_RS07525 (AA961_07525) comGC 1529294..1529605 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AA961_RS07530 (AA961_07530) comGB 1529619..1530689 (-) 1071 WP_047928470.1 competence type IV pilus assembly protein ComGB Machinery gene
  AA961_RS07535 (AA961_07535) comGA 1530661..1531635 (-) 975 WP_001633882.1 competence type IV pilus ATPase ComGA Machinery gene
  AA961_RS07540 (AA961_07540) - 1531687..1532310 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AA961_RS07545 (AA961_07545) - 1532307..1532636 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AA961_RS07550 (AA961_07550) - 1532636..1533622 (-) 987 WP_047928471.1 ROK family glucokinase -
  AA961_RS07555 (AA961_07555) - 1533619..1533822 (-) 204 WP_000087561.1 YqgQ family protein -
  AA961_RS07560 (AA961_07560) - 1533803..1535266 (-) 1464 WP_001017858.1 rhomboid family protein -
  AA961_RS07565 (AA961_07565) - 1535278..1535817 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  AA961_RS07570 (AA961_07570) rpmG 1536026..1536175 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36898.90 Da        Isoelectric Point: 8.4460

>NTDB_id=146647 AA961_RS07535 WP_001633882.1 1530661..1531635(-) (comGA) [Staphylococcus aureus strain RKI4]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFENLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=146647 AA961_RS07535 WP_001633882.1 1530661..1531635(-) (comGA) [Staphylococcus aureus strain RKI4]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTGAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment