Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   DHB4_RS18475 Genome accession   NZ_CP014270
Coordinates   3716130..3716798 (-) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli K-12 strain K-12 DHB4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3711130..3721798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DHB4_RS18445 (DHB4_18470) rfaQ 3711303..3712373 (-) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -
  DHB4_RS18450 (DHB4_18475) waaA 3712779..3714056 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  DHB4_RS18455 (DHB4_18480) coaD 3714064..3714543 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  DHB4_RS18460 (DHB4_18485) mutM 3714582..3715391 (-) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  DHB4_RS18465 (DHB4_18490) rpmG 3715489..3715656 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  DHB4_RS18470 (DHB4_18495) rpmB 3715677..3715913 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  DHB4_RS18475 (DHB4_18500) radC 3716130..3716798 (-) 669 WP_001350561.1 RadC family protein Machinery gene
  DHB4_RS18480 (DHB4_18505) coaBC 3716970..3718190 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  DHB4_RS18485 (DHB4_18510) dut 3718168..3718626 (+) 459 WP_000976073.1 dUTP diphosphatase -
  DHB4_RS18490 (DHB4_18515) slmA 3718733..3719329 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  DHB4_RS18495 (DHB4_18520) pyrE 3719366..3720007 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  DHB4_RS18500 (DHB4_18525) rph 3720073..3720789 (-) 717 WP_001247096.1 ribonuclease PH -
  DHB4_RS18505 (DHB4_18530) yicC 3720916..3721779 (+) 864 WP_000621340.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=145201 DHB4_RS18475 WP_001350561.1 3716130..3716798(-) (radC) [Escherichia coli K-12 strain K-12 DHB4]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=145201 DHB4_RS18475 WP_001350561.1 3716130..3716798(-) (radC) [Escherichia coli K-12 strain K-12 DHB4]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment